Isolation and characterization of microsatellite loci in Palinurus elephas

被引：4
|
作者：
Palero, F. [1 ]
Pascual, M. [1 ]
机构：
[1] Univ Barcelona, Fac Biol, Dept Genet, E-08028 Barcelona, Spain
来源：
MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 06期
关键词：
conservation genetics; crustacea; gene flow; microsatellites; Palinurus;
D O I：
10.1111/j.1755-0998.2008.02230.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The European spiny lobster (Palinurus elephas) mean annual catches have decreased alarmingly during recent decades along its entire distribution area due to stock over-exploitation, which makes it a primary target for conservation plans. A total of 164 microsatellite loci were isolated from a genomic library of P. elephas enriched for CA, GA, CAA and GATA repeats. A total of 15 polymorphic loci have been screened in 48 individuals. High numbers of alleles per locus (averaging 20 +/- 10.5) and observed heterozygosity (averaging 0.789 +/- 0.197) have been detected. None of the pairs of loci showed significant linkage disequilibrium. Two of the loci (Pael1 and Pael2) showed significant departure from Hardy-Weinberg equilibrium in Sagres, while Pael38 showed significant departure in Tunis. These highly polymorphic markers will be useful in determining the spatial patterns of genetic diversity between and within populations of Palinurus elephas.
引用
收藏
页码：1477 / 1479
页数：3
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