Isolation and characterization of microsatellite loci in Onychostoma sima

被引：0
|
作者：
Meihua Xiong
Yanfu Que
Fang Shi
Nian Xu
Zhong Yang
Jianbo Chang
Juxiang Hu
Bin Zhu
机构：
[1] Ministry of Water Resources and Chinese Academy of Sciences,Institute of Hydroecology
来源：
Conservation Genetics Resources | 2009年 / 1卷
关键词：
 ; Microsatellite; Polymorphism;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Seventeen polymorphic microsatellite loci have been isolated from an (AAAG)n enriched genomic library of Onychostoma sima. These loci were tested against 32 wild captured individuals for polymorphism. The number of observed alleles per locus ranged from 2 to 10. The expected and observed heterozygosity values ranged from 0.25 to 0.96 and from 0.26 to 0.87, respectively. Seven loci showed significant deviation from Hardy–Weinberg Equilibrium and linkage equilibrium were detected in three loci. These polymorphic loci described for O. sima will be valuable as genetic markers for use in population structure, breeding, and conservation studies.
引用
收藏
相关论文
共 50 条
  • [1] Isolation and characterization of microsatellite loci in Onychostoma sima
    Xiong, Meihua
    Que, Yanfu
    Shi, Fang
    Xu, Nian
    Yang, Zhong
    Chang, Jianbo
    Hu, Juxiang
    Zhu, Bin
    CONSERVATION GENETICS RESOURCES, 2009, 1 (01) : 389 - 392
  • [2] Isolation and characterization of microsatellite loci in Fragaria
    Cipriani, G
    Testolin, R
    MOLECULAR ECOLOGY NOTES, 2004, 4 (03): : 366 - 368
  • [3] Isolation and Characterization of the Bovine Microsatellite Loci
    H. Y. Chung
    T. H. Kim
    B. H. Choi
    G. W. Jang
    J. W. Lee
    K. T. Lee
    J. M. Ha
    Biochemical Genetics, 2006, 44 : 518 - 532
  • [4] Isolation and characterization of microsatellite loci in Senecio
    Liu, GQ
    Hegarty, MJ
    Edwards, KJ
    Hiscock, SJ
    Abbott, RJ
    MOLECULAR ECOLOGY NOTES, 2004, 4 (04): : 611 - 614
  • [5] Isolation and characterization of the bovine microsatellite loci
    Chung, H. Y.
    Kim, T. H.
    Choi, B. H.
    Jang, G. W.
    Lee, J. W.
    Lee, K. T.
    Ha, J. M.
    BIOCHEMICAL GENETICS, 2006, 44 (11-12) : 527 - 541
  • [6] Isolation and characterization of microsatellite loci in Jinshaia sinensis
    Shao, Ke
    Shi, Fang
    Tang, Huiyuan
    Xiong, Meihua
    Li, Weitao
    Zhu, Bin
    CONSERVATION GENETICS RESOURCES, 2012, 4 (04) : 1075 - 1077
  • [7] Isolation and characterization of microsatellite loci in Bemisia tabaci
    De Barro, PJ
    Scott, KD
    Graham, GC
    Lange, CL
    Schutze, MK
    MOLECULAR ECOLOGY NOTES, 2003, 3 (01): : 40 - 43
  • [8] Isolation and characterization of microsatellite loci in Tripterygion delaisi
    Carreras-Carbonell, J
    MacPherson, E
    Pascual, M
    MOLECULAR ECOLOGY NOTES, 2004, 4 (03): : 438 - 439
  • [9] Isolation and characterization of microsatellite loci in Rhinogobio ventralis
    Xu, Nian
    Shi, Fang
    Xiong, Meihua
    Que, Yanfu
    Yang, Zhong
    Zhu, Bin
    Chang, Jianbo
    CONSERVATION GENETICS RESOURCES, 2010, 2 : 1 - 3
  • [10] Isolation and characterization of microsatellite loci from Macadamia
    Schmidt, A. L.
    Scott, L.
    Lowe, A. J.
    MOLECULAR ECOLOGY NOTES, 2006, 6 (04): : 1060 - 1063
12345