The 5′ untranslated region of Apaf-1 mRNA directs translation under apoptosis conditions via a 5′ end-dependent scanning mechanism

被引：24
|
作者：
Andreev, Dmitri E. [1 ]
Dmitriev, Sergey E. [1 ]
Zinovkin, Roman [2 ]
Terenin, Ilya M. [1 ]
Shatsky, Ivan N. [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Moscow 119234, Russia
[2] Moscow MV Lomonosov State Univ, Inst Mitoengn, Moscow 119234, Russia
来源：
FEBS LETTERS | 2012年 / 586卷 / 23期
基金：
俄罗斯基础研究基金会;
关键词：
Translation control; Internal ribosome entry site (IRES); Cap-independent translation enhancer (CITE); Apoptotic peptidase activating factor 1 (Apaf-1); Etoposide; INTERNAL RIBOSOME ENTRY; IRES-MEDIATED TRANSLATION; INITIATION; STRESS; CAP; SITE; SEGMENT; CANCER; EIF4G;
D O I：
10.1016/j.febslet.2012.10.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have previously shown that translation driven by the 5' UTR of Apaf-1 mRNA is relatively efficient in the absence of m7G-cap, but no IRES is involved. Nevertheless, it may be speculated that a "silent" IRES is activated under apoptosis conditions. Here, we show that translation of the mRNA with the Apaf-1 5' UTR is relatively resistant to apoptosis induced by etoposide when eIF4E is sequestered by 4E-BP and eIF4G is partially cleaved. However, translation under these conditions remains governed by 5' end-dependent scanning. We hypothesize that the observed phenomenon is based on the intrinsic low cap-dependence of the Apaf-1 5' UTR. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码：4139 / 4143
页数：5
相关论文
共 50 条
  • [41] Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation
    Gan, WN
    Rhoads, RE
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (02) : 623 - 626
  • [42] Insulin growth factor 2 mRNA binding protein 1 (IGF2BP1) regulates translation of the multidrug resistance protein 2 (MRP2) by binding to its 5′-untranslated region (5′UTR)
    Vore, Mary
    Zhao, Tianyong
    Zhang, Yanbin
    Yan, Baoxiang
    Thompson, Sunnie R.
    FASEB JOURNAL, 2011, 25
  • [43] Translation of a testis-specific Cu/Zn superoxide dismutase (SOD-1) mRNA is regulated by a 65-kilodalton protein which binds to its 5' untranslated region
    Gu, W
    Hecht, NB
    MOLECULAR AND CELLULAR BIOLOGY, 1996, 16 (08) : 4535 - 4543
  • [44] Truncated form of VACM-1/cul-5 with an extended 3′ untranslated region stimulates cell growth via a MAPK-dependent pathway
    Sartor, A
    Kossoris, JB
    Wilcox, R
    Shearer, R
    Zeneberg, AE
    Zhao, P
    Lazidins, I
    Burnatowska-Hledin, MA
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 343 (04) : 1086 - 1093
  • [45] Internal initiation of translation directed by the 5 prime -untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from Cap-dependent to internal initiation
    J. Biol. Chem., 2 (623-626):
  • [46] 1,25-dihydroxyvitamin D3 may stabilize osteocalcin mRNA via the predicted stem-loop structure in the 5-untranslated region.
    Watana, S
    Mosavin, R
    Mellon, WS
    FASEB JOURNAL, 2000, 14 (08): : A1570 - A1570
  • [47] Novel 5′ Untranslated Region Directed Blockers of Iron-Regulatory Protein-1 Dependent Amyloid Precursor Protein Translation: Implications for Down Syndrome and Alzheimer's Disease
    Bandyopadhyay, Sanghamitra
    Cahill, Catherine
    Balleidier, Amelie
    Huang, Conan
    Lahiri, Debomoy K.
    Huang, Xudong
    Rogers, Jack T.
    PLOS ONE, 2013, 8 (07):
  • [48] Yersinia enterocolitica type III secretion:: yscM1 and yscM2 regulate yop gene expression by a posttranscriptional mechanism that targets the 5′ untranslated region of yop mRNA
    Cambronne, ED
    Schneewind, O
    JOURNAL OF BACTERIOLOGY, 2002, 184 (21) : 5880 - 5893
  • [49] Efficient translation initiation directed by the 900-nucleotide-long and GC-rich 5′ untranslated region of the human retrotransposon LINE-1 mRNA is strictly cap dependent rather than internal ribosome entry site mediated
    Dmitriev, Sergey E.
    Andreev, Dmitri E.
    Terenin, Ilya M.
    Olovnikov, Ivan A.
    Prassolov, Vladimir S.
    Merrick, William C.
    Shatsky, Ivan N.
    MOLECULAR AND CELLULAR BIOLOGY, 2007, 27 (13) : 4685 - 4697
  • [50] RNA-binding protein HuR interacts with thrombomodulin 5′untranslated region and represses internal ribosome entry site-mediated translation under IL-1β treatment
    Yeh, Chiu-Hung
    Hung, Liang-Yi
    Hsu, Chin
    Le, Shu-Yun
    Lee, Pin-Tse
    Liao, Wan-Lin
    Lin, Yi-Tseng
    Chang, Wen-Chang
    Tseng, Joseph T.
    MOLECULAR BIOLOGY OF THE CELL, 2008, 19 (09) : 3812 - 3822
12345