The 5′ untranslated region of Apaf-1 mRNA directs translation under apoptosis conditions via a 5′ end-dependent scanning mechanism

被引：24
|
作者：
Andreev, Dmitri E. [1 ]
Dmitriev, Sergey E. [1 ]
Zinovkin, Roman [2 ]
Terenin, Ilya M. [1 ]
Shatsky, Ivan N. [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Moscow 119234, Russia
[2] Moscow MV Lomonosov State Univ, Inst Mitoengn, Moscow 119234, Russia
来源：
FEBS LETTERS | 2012年 / 586卷 / 23期
基金：
俄罗斯基础研究基金会;
关键词：
Translation control; Internal ribosome entry site (IRES); Cap-independent translation enhancer (CITE); Apoptotic peptidase activating factor 1 (Apaf-1); Etoposide; INTERNAL RIBOSOME ENTRY; IRES-MEDIATED TRANSLATION; INITIATION; STRESS; CAP; SITE; SEGMENT; CANCER; EIF4G;
D O I：
10.1016/j.febslet.2012.10.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have previously shown that translation driven by the 5' UTR of Apaf-1 mRNA is relatively efficient in the absence of m7G-cap, but no IRES is involved. Nevertheless, it may be speculated that a "silent" IRES is activated under apoptosis conditions. Here, we show that translation of the mRNA with the Apaf-1 5' UTR is relatively resistant to apoptosis induced by etoposide when eIF4E is sequestered by 4E-BP and eIF4G is partially cleaved. However, translation under these conditions remains governed by 5' end-dependent scanning. We hypothesize that the observed phenomenon is based on the intrinsic low cap-dependence of the Apaf-1 5' UTR. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码：4139 / 4143
页数：5
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