Molecular Weight and Monosaccharide Composition of Astragalus Polysaccharides

被引：54
|
作者：
Xu, Du-Juan [1 ]
Xia, Quan [1 ]
Wang, Jia-Jia [2 ]
Wang, Pei-Pei [2 ]
机构：
[1] Anhui Med Univ, Affiliated Hosp 1, Dept Pharm, Hefei 230022, Peoples R China
[2] Anhui Med Univ, Sch Pharm, Hefei 230032, Peoples R China
来源：
MOLECULES | 2008年 / 13卷 / 10期
关键词：
Astragalus polysaccharides; Isolation and Purification; Molecular Weight; Monosaccharide composition;
D O I：
10.3390/molecules13102408
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLCGPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH2 column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1: 3.45 and 1: 6.25: 17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively.
引用
收藏
页码：2408 / 2415
页数：8
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