Molecular Weight and Monosaccharide Composition of Astragalus Polysaccharides

被引：54
|
作者：
Xu, Du-Juan [1 ]
Xia, Quan [1 ]
Wang, Jia-Jia [2 ]
Wang, Pei-Pei [2 ]
机构：
[1] Anhui Med Univ, Affiliated Hosp 1, Dept Pharm, Hefei 230022, Peoples R China
[2] Anhui Med Univ, Sch Pharm, Hefei 230032, Peoples R China
来源：
MOLECULES | 2008年 / 13卷 / 10期
关键词：
Astragalus polysaccharides; Isolation and Purification; Molecular Weight; Monosaccharide composition;
D O I：
10.3390/molecules13102408
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLCGPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH2 column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1: 3.45 and 1: 6.25: 17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively.
引用
收藏
页码：2408 / 2415
页数：8
相关论文
共 50 条
  • [21] Composition and Detection of Monosaccharide in the Polysaccharides of Juglans sigillata D. Shells
    Haizhu ZHANG
    Xi LIU
    Ping ZHOU
    Zhiting SHI
    Shuilai DENG
    Hai ZHANG
    Yuan MA
    Medicinal Plant, 2013, (07) : 12 - 15
  • [22] Determination of molecular weights and monosaccharide compositions in Abelmoschus manihot polysaccharides
    Wang, Xuemei
    Wang, Yingying
    Wu, Mimi
    Zhang, Xiuzhen
    RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY A, 2012, 86 (09) : 1469 - 1472
  • [23] MONOSACCHARIDE COMPOSITION OF SURFACE-LOCALIZED POLYSACCHARIDES OF PEA NODULE BACTERIA
    KOSENKO, LV
    PATSEVA, MA
    ZAKHAROVA, IY
    MICROBIOLOGY, 1990, 59 (02) : 191 - 196
  • [24] Molecular Weight (Mw) and Monosaccharide Composition (MC): Two Major Factors Affecting the Therapeutic Action of Polysaccharides Extracted from Cordyceps sinensis-Mini Review
    Soltani, Mohammad
    Kamyab, Hesam
    El-Enshasy, Hesham A.
    JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2013, 7 (03): : 1601 - 1613
  • [25] A rapid-throughput adaptable method for determining the monosaccharide composition of polysaccharides
    Amicucci, Matthew J.
    Galermo, Ace G.
    Nandita, Eshani
    Vo, Thai-Thanh T.
    Liu, Yiyun
    Lee, Megan
    Xu, Gege
    Lebrilla, Carlito B.
    INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2019, 438 : 22 - 28
  • [26] EFFECT OF ISOLATION PROCEDURE ON MOLECULAR-WEIGHT DISTRIBUTION AND MONOSACCHARIDE COMPOSITION OF COTTON STALK LIGNINS
    YOSEF, E
    BENGHEDALIA, D
    ANIMAL FEED SCIENCE AND TECHNOLOGY, 1994, 50 (1-2) : 27 - 35
  • [27] The molecular weight of specific polysaccharides
    Heidelberger, M
    Kendall, FE
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1932, 96 (02) : 541 - 558
  • [28] Isolation, Purification, Monosaccharide Composition and Antioxidant Activity of Polysaccharides from Cortex Periplocae
    Ruiping Z.
    Zhaohui R.
    Haonan Z.
    Xiaoping Z.
    Pengfei L.
    Xiaoli W.
    Science and Technology of Food Industry, 2023, 44 (13) : 71 - 78
  • [29] MONOSACCHARIDE COMPOSITION OF POLYSACCHARIDES THROUGH HILIC-UPLC-TQ-MS/MS
    Yong-Gang Xia
    Li-Ming Sun
    Jun Liang
    Qiu-Hong Wang
    Hai-Xue Kuang
    World Journal of Traditional Chinese Medicine, 2015, (04) : 92 - 92
  • [30] Analysis of the Monosaccharide Composition of Purified Polysaccharides in Ganoderma atrum by Capillary Gas Chromatography
    Chen, Yi
    Xie, Ming-Yong
    Wang, Yuan-Xing
    Nie, Shao-Ping
    Li, Chang
    PHYTOCHEMICAL ANALYSIS, 2009, 20 (06) : 503 - 510
12345