Rabbit ubiquitin-activating enzyme E1: CDNA cloning, sequence and expression

被引：7
|
作者：
Sun, BG [1 ]
Jeyaseelan, K [1 ]
Chung, MCM [1 ]
Teo, TS [1 ]
机构：
[1] NATL UNIV SINGAPORE,FAC MED,DEPT BIOCHEM,SINGAPORE 119260,SINGAPORE
来源：
GENE | 1997年 / 196卷 / 1-2期
关键词：
ubiquitination; protein degradation; tissue distribution;
D O I：
10.1016/S0378-1119(97)00154-6
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A cDNA clone encoding ubiquitin-activating enzyme E1 has been isolated from a rabbit heart cDNA library and sequenced. The 3.485 kb cDNA contains an open reading frame of 1058 amino acid residues which predicts a protein of approx. 118 kDa. The deduced protein sequence exhibits a very high homology to other ubiquitin-activating enzymes identified in a variety of organisms. Northern blot analysis reveals a single transcript of apporx. 3.5 kb in all the rabbit tissues examined. The entire coding region of the rabbit E1 cDNA has been expressed as a his-tagged protein. The recombinant protein has been verified by its ability to cross-react with anti-human E1 antibodies. Ubiquitin thiolester assay shows that the recombinant rabbit E1 protein is functional. (C) 1997 Elsevier Science B.V.
引用
收藏
页码：19 / 23
页数：5
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