Rabbit ubiquitin-activating enzyme E1: CDNA cloning, sequence and expression

被引：7
|
作者：
Sun, BG [1 ]
Jeyaseelan, K [1 ]
Chung, MCM [1 ]
Teo, TS [1 ]
机构：
[1] NATL UNIV SINGAPORE,FAC MED,DEPT BIOCHEM,SINGAPORE 119260,SINGAPORE
来源：
GENE | 1997年 / 196卷 / 1-2期
关键词：
ubiquitination; protein degradation; tissue distribution;
D O I：
10.1016/S0378-1119(97)00154-6
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A cDNA clone encoding ubiquitin-activating enzyme E1 has been isolated from a rabbit heart cDNA library and sequenced. The 3.485 kb cDNA contains an open reading frame of 1058 amino acid residues which predicts a protein of approx. 118 kDa. The deduced protein sequence exhibits a very high homology to other ubiquitin-activating enzymes identified in a variety of organisms. Northern blot analysis reveals a single transcript of apporx. 3.5 kb in all the rabbit tissues examined. The entire coding region of the rabbit E1 cDNA has been expressed as a his-tagged protein. The recombinant protein has been verified by its ability to cross-react with anti-human E1 antibodies. Ubiquitin thiolester assay shows that the recombinant rabbit E1 protein is functional. (C) 1997 Elsevier Science B.V.
引用
收藏
页码：19 / 23
页数：5
相关论文
共 50 条
  • [31] PYR-41, A UBIQUITIN-ACTIVATING ENZYME E1 INHIBITOR, ATTENUATES ORGAN INJURY IN SEPSIS
    Matsuo, S.
    Yang, W.
    Wang, P.
    SHOCK, 2013, 39 : 17 - 18
  • [32] The ubiquitin-activating enzyme E1 is phosphorylated and localized to the nucleus in a cell cycle-dependent manner
    Stephen, AG
    TrauschAzar, JS
    Ciechanover, A
    Schwartz, AL
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (26) : 15608 - 15614
  • [33] Impaired nucleotide excision repair upon macrophage differentiation is corrected by E1 ubiquitin-activating enzyme
    Nouspikel, Thierry
    Hanawalt, Philip C.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (44) : 16188 - 16193
  • [34] Steady-State Kinetic Analysis of Human Ubiquitin-Activating Enzyme (E1) Using a Fluorescently Labeled Ubiquitin Substrate
    Kevin E. Wee
    Zhihong Lai
    Kurt R. Auger
    Jianhong Ma
    Kurumi Y. Horiuchi
    Randine L. Dowling
    Cristy S. Dougherty
    Jeanne I. Corman
    Richard Wynn
    Robert A. Copeland
    Journal of Protein Chemistry, 2000, 19 (6): : 489 - 498
  • [35] UBIQUITIN-DEPENDENT PROTEOLYTIC PATHWAY IN WHEAT-GERM - ISOLATION OF MULTIPLE FORMS OF UBIQUITIN-ACTIVATING ENZYME, E1
    HATFIELD, PM
    VIERSTRA, RD
    BIOCHEMISTRY, 1989, 28 (02) : 735 - 742
  • [36] Steady-state kinetic analysis of human ubiquitin-activating enzyme (E1) using a fluorescently labeled ubiquitin substrate
    Wee, KE
    Lai, ZH
    Auger, KR
    Ma, JH
    Horiuchi, KY
    Dowling, RL
    Dougherty, CS
    Corman, JI
    Wynn, R
    Copeland, RA
    JOURNAL OF PROTEIN CHEMISTRY, 2000, 19 (06): : 489 - 498
  • [37] Ubiquitin-Activating Enzyme E1 Inhibition Caused Acute Leukemia Cell Apoptosis By Affecting CHOP Pathway
    Bai, Ju
    He, Aili
    Wang, Jianli
    Yang, Yun
    Shen, Ying
    Feng, Yuandong
    Xu, Yan
    BLOOD, 2018, 132
  • [38] MULTIPLE FORMS OF UBIQUITIN-ACTIVATING ENZYME E1 FROM WHEAT - IDENTIFICATION OF AN ESSENTIAL CYSTEINE BY INVITRO MUTAGENESIS
    HATFIELD, PM
    VIERSTRA, RD
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1992, 267 (21) : 14799 - 14803
  • [39] The E1 ubiquitin-activating enzyme Uba1 in Drosophila controls apoptosis autonomously and tissue growth nonautonomously
    Lee, Tom V.
    Ding, Tian
    Chen, Zhihong
    Rajendran, Vani
    Scherr, Heather
    Lackey, Melinda
    Bolduc, Clare
    Bergmann, Andreas
    DEVELOPMENT, 2008, 135 (01): : 43 - 52
  • [40] A Temperature-sensitive Mutation in the Ubiquitin-activating Enzyme E1 and its Relation to the Maintenance of Chromosome Integrity
    Sugaya, K.
    Ishihara, Y.
    Inoue, S.
    Tsuji, H.
    MOLECULAR BIOLOGY OF THE CELL, 2012, 23
12345