Cytological aspects and anthocyanin accumulation observed in Sedum telephium ssp maximum L. callus

被引：0
|
作者：
Ardelean, Mirela [1 ]
Cachita-Cosma, Dorina [1 ]
Ardelean, Aurel [1 ]
Ladasiu, Flaviu-Calin [1 ]
Lobiuc, Andrei [2 ,3 ]
Zamfirache, Maria-Magdalena [2 ]
Rosenhech, Elida [2 ]
机构：
[1] Vasile Goldis Western Univ Arad, Inst Life Sci, Plant Biotechnol, Arad, Romania
[2] Stefan Cel Mare Univ, Fac Food Engn, Univ Str 13, Suceava, Romania
[3] Alexandru Ioan Cuza Univ, CERNESIM, Fac Biol, Carol I Bd, Iasi, Romania
来源：
ROMANIAN BIOTECHNOLOGICAL LETTERS | 2017年 / 22卷 / 06期
关键词：
cyanidin; 3-glucoside; growth regulators; callusogenesis; CULTURES; GROWTH;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Sedum telephium ssp. maximum L. calluses grown on Murashige-Skoog growth media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BAP) showed a red coloration of the vacuolar content of some of their cells. It was determined that this phenomenon is due to anthocyanins accumulation under the influence of the growth regulators. Using HPLC analysis, it was found that the highest overall anthocyanins concentration was in calluses grown on medium supplemented with 1.5mg/l 2,4-D + 2.5 mg/l BAP. Furthermore, the type of the growth regulators (cytokinines or auxines) added individually or as a mixture (in different ratios) in the growth medium, can influence the callus growth rate and what type of anthocyanins is produced preferentially.
引用
收藏
页码：13125 / 13134
页数：10
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