Isolation and characterization of collagen from the skin of Brama australis

被引：41

作者：

Sionkowska, Alina ^{[1
]}

Kozlowska, Justyna ^{[1
]}

Skorupska, Malgorzata ^{[1
]}

Michalska, Marta ^{[1
]}

机构：

[1] Nicolaus Copernicus Univ, Fac Chem, Dept Chem Biomat & Cosmet, PL-87100 Torun, Poland

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 80卷

关键词：

Collagen; Fish skin; Denaturation temperature; ACID-SOLUBLE COLLAGEN; FISH; PROTEINS; SCALE;

D O I：

10.1016/j.ijbiomac.2015.07.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Collagen was extracted from the skin of Brama australis, the fish from warm-water sea. The yield of collagen from skin of B. australis was about 1.5% on a wet weight basis of raw material. The isolated protein was confirmed as collagen by different physico-chemical techniques such as: FTIR, SOS-PAGE, and amino acid analysis. The denaturation temperature (T-d) of obtained collagen was found to be 24 degrees C, what is promising as an advantage for cosmetic application. According to the electrophoretic pattern, collagen consisted of two different alpha-chains (alpha 1 and alpha 2) and was classified as type I collagen. Although T-d of obtained collagen is higher than 20 degrees C it is still far from T-d of mammalian collagen. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：605 / 609

页数：5

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