Isolation and characterization of collagen from the skin of Brama australis

Collagen was extracted from the skin of Brama australis, the fish from warm-water sea. The yield of collagen from skin of B. australis was about 1.5% on a wet weight basis of raw material. The isolated protein was confirmed as collagen by different physico-chemical techniques such as: FTIR, SOS-PAGE, and amino acid analysis. The denaturation temperature (T-d) of obtained collagen was found to be 24 degrees C, what is promising as an advantage for cosmetic application. According to the electrophoretic pattern, collagen consisted of two different alpha-chains (alpha 1 and alpha 2) and was classified as type I collagen. Although T-d of obtained collagen is higher than 20 degrees C it is still far from T-d of mammalian collagen. (C) 2015 Elsevier B.V. All rights reserved.