Comparison of Restriction Enzymes for Pulsed-Field Gel Electrophoresis Typing of Moraxella catarrhalis

被引：6

作者：

Marti, Sara ^{[1
,2
]}

Puig, Carmen ^{[1
,2
]}

Domenech, Arnau ^{[1
,2
]}

Linares, Josefina ^{[1
,2
]}

Ardanuy, Carmen ^{[1
,2
]}

机构：

[1] Univ Barcelona, Hosp Univ Bellvitge, IDIBELL, Dept Microbiol, Barcelona, Spain

[2] ISCIII, CIBER Enfermedades Resp, Madrid, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 07期

关键词：

RESPIRATORY-TRACT PATHOGEN; MOLECULAR ASPECTS; DNA RESTRICTION; INFECTION; GENOMES; COPD;

D O I：

10.1128/JCM.00823-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

NotI, the most prevalent restriction enzyme used for typing Moraxella catarrhalis, failed to digest genomic DNA from respiratory samples. An improved pulsed-field gel electrophoresis (PFGE) methodology determined SpeI as the best choice for typing this bacterial species, with a good restriction of clinical samples and a good clustering correlation with NotI.

引用

页码：2448 / 2452

页数：5

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