Comparison of Restriction Enzymes for Pulsed-Field Gel Electrophoresis Typing of Moraxella catarrhalis

被引：6
|
作者：
Marti, Sara [1 ,2 ]
Puig, Carmen [1 ,2 ]
Domenech, Arnau [1 ,2 ]
Linares, Josefina [1 ,2 ]
Ardanuy, Carmen [1 ,2 ]
机构：
[1] Univ Barcelona, Hosp Univ Bellvitge, IDIBELL, Dept Microbiol, Barcelona, Spain
[2] ISCIII, CIBER Enfermedades Resp, Madrid, Spain
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 07期
关键词：
RESPIRATORY-TRACT PATHOGEN; MOLECULAR ASPECTS; DNA RESTRICTION; INFECTION; GENOMES; COPD;
D O I：
10.1128/JCM.00823-13
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
NotI, the most prevalent restriction enzyme used for typing Moraxella catarrhalis, failed to digest genomic DNA from respiratory samples. An improved pulsed-field gel electrophoresis (PFGE) methodology determined SpeI as the best choice for typing this bacterial species, with a good restriction of clinical samples and a good clustering correlation with NotI.
引用
收藏
页码：2448 / 2452
页数：5
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