Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization

被引：20
|
作者：
Chae, Pil Seok [1 ]
Wander, Marc J. [2 ]
Cho, Kyung Ho [1 ]
Laible, Philip D. [2 ]
Gellman, Samuel H. [3 ]
机构：
[1] Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea
[2] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA
[3] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA
来源：
MOLECULAR BIOSYSTEMS | 2013年 / 9卷 / 04期
基金：
新加坡国家研究基金会;
关键词：
MUSCARINIC ACETYLCHOLINE-RECEPTOR; FACIAL AMPHIPHILES; AQUEOUS-SOLUTIONS; CARBON NANOTUBES; DETERGENT; SURFACTANTS; CRYSTALLIZATION; ADRENOCEPTOR; ANTAGONIST; AMPHIPOLS;
D O I：
10.1039/c3mb25584k
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Membrane protein manipulation is a challenging task owing to limited tertiary and quaternary structural stability once the protein has been removed from a lipid bilayer. Such instability can be overcome by embedding membrane proteins in detergent micelles formed from amphiphiles with carefully tuned properties. This study introduces a class of easy-to-synthesize amphiphiles, which are designated CGT (Chae's Glyco-Triton) detergents. Some of the agents are well suited for membrane protein solubilization and stabilization.
引用
收藏
页码：626 / 629
页数：4
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