Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization

被引：20

作者：

Chae, Pil Seok ^{[1
]}

Wander, Marc J. ^{[2
]}

Cho, Kyung Ho ^{[1
]}

Laible, Philip D. ^{[2
]}

Gellman, Samuel H. ^{[3
]}

机构：

[1] Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea

[2] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA

[3] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA

来源：

MOLECULAR BIOSYSTEMS | 2013年 / 9卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

MUSCARINIC ACETYLCHOLINE-RECEPTOR; FACIAL AMPHIPHILES; AQUEOUS-SOLUTIONS; CARBON NANOTUBES; DETERGENT; SURFACTANTS; CRYSTALLIZATION; ADRENOCEPTOR; ANTAGONIST; AMPHIPOLS;

D O I：

10.1039/c3mb25584k

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Membrane protein manipulation is a challenging task owing to limited tertiary and quaternary structural stability once the protein has been removed from a lipid bilayer. Such instability can be overcome by embedding membrane proteins in detergent micelles formed from amphiphiles with carefully tuned properties. This study introduces a class of easy-to-synthesize amphiphiles, which are designated CGT (Chae's Glyco-Triton) detergents. Some of the agents are well suited for membrane protein solubilization and stabilization.

引用

页码：626 / 629

页数：4

共 50 条

[21] LOWRY DETERMINATION OF PROTEIN IN PRESENCE OF TRITON X-100
WANG, CS
SMITH, RL
ANALYTICAL BIOCHEMISTRY, 1975, 63 (02) : 414 - 417
[22] PHOSVITIN RECEPTOR FROM CHICKEN OOCYTES - EVIDENCE FOR SOLUBILIZATION BY TRITON X-100
WOODS, JW
ROTH, TF
JOURNAL OF SUPRAMOLECULAR STRUCTURE, 1979, : 119 - 119
[23] SOLUBILIZATION AND PROPERTIES OF GALACTOSYLTRANSFERASE AND SULFOTRANSFERASE ACTIVITIES OF GOLGI MEMBRANES IN TRITON X-100
FLEISCHER, B
SMIGEL, M
JOURNAL OF BIOLOGICAL CHEMISTRY, 1978, 253 (05) : 1632 - 1638
[24] Assembly properties of triton X-100/phosphatidylcholine aggregates during liposome solubilization
delaMaza, A
Parra, JL
COLLOID AND POLYMER SCIENCE, 1996, 274 (09) : 866 - 874
[25] Specific features of the solubilization of multinuclear aromatic hydrocarbons in triton X-100 micelles
I. A. Ar’ev
N. A. Klimenko
L. V. Nevinnaya
A. V. Russu
Colloid Journal, 2006, 68 : 529 - 532
[26] EFFECTIVE DETERGENT LIPID RATIOS IN THE SOLUBILIZATION OF PHOSPHATIDYLCHOLINE VESICLES BY TRITON X-100
PARTEARROYO, MA
URBANEJA, MA
GONI, FM
FEBS LETTERS, 1992, 302 (02) : 138 - 140
[27] SOLUBILIZATION OF MITOCHONDRIAL HEXOKINASE FROM OX CEREBRAL CORTEX WITH TRITON X-100
THOMPSON, MF
BACHELARD, HS
BIOCHEMICAL JOURNAL, 1969, 111 (03) : P18 - +
[28] Direct formation of mixed micelles in the solubilization of phospholipid liposomes by Triton X-100
López, O
de la Maza, A
Coderch, L
López-Iglesias, C
Wehrli, E
Parra, JL
FEBS LETTERS, 1998, 426 (03) : 314 - 318
[29] ACTIVATION AND SOLUBILIZATION BY TRITON X-100 OF MEMBRANE-BOUND PHOSPHOLIPASE-D OF RAT-BRAIN
KANOH, H
KANAHO, Y
NOZAWA, Y
LIPIDS, 1991, 26 (06) : 426 - 430
[30] Stabilization and desestabilization of model colloids after adsorption of Triton X-100
Romero-Cano, MS
Martín-Rodríguez, A
De las Nieves, FJ
BOLETIN DE LA SOCIEDAD ESPANOLA DE CERAMICA Y VIDRIO, 2000, 39 (04): : 445 - 447

← 1 2 3 4 5 →