Li+ effect on the cell wall of the yeast Saccharomyces cerevisiae as probed by FT-IR spectroscopy

被引：9

作者：

Zimkus, Aurelijus ^{[1
]}

Misiunas, Audrius ^{[2
]}

Chaustova, Larisa ^{[3
]}

机构：

[1] Vilnius State Univ, Dept Biochem & Biophys, LT-01513 Vilnius, Lithuania

[2] Ctr Phys Sci & Technol, Dept Organ Chem, LT-01108 Vilnius, Lithuania

[3] Vilnius State Univ, Inst Biochem, Dept Bioelectrochem & Biospectroscopy, LT-01513 Vilnius, Lithuania

来源：

CENTRAL EUROPEAN JOURNAL OF BIOLOGY | 2013年 / 8卷 / 08期

关键词：

FT-IR spectroscopy; Lithium; Saccharomyces cerevisiae; Transformation; MANNAN STRUCTURE; TRANSFORMATION; DNA; IDENTIFICATION; DYNAMICS; CANDIDA; WATER;

D O I：

10.2478/s11535-013-0186-1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effect of Li+ ions as a transformation inducing agent on the yeast cell wall has been studied. Two Saccharomyces cerevisiae strains, p63-DC5 with a native cell wall, and strain XCY42-30D(mnn1) which contains structural changes in the mannan-protein complex, were used. Fourier transform infrared (FT-IR) spectroscopy has been used for the characterization of the yeast strains and for determination of the effect of lithium cations on the cell wall. A comparison of the carbohydrate absorption band positions in the 970-1185 cm(-1) range, of Na+ and Li+ treated yeast cells has been estimated. Absorption band positions of the cell wall carbohydrates of p63-DC5 were not influenced by the studied ions. On the contrary, the treatment of XCY42-30D(mnn1) cells with Li+ ions shifted glucan band positions, implying that the cell wall structure of strain XCY42-30D(mnn1) is more sensitive to Li+ ion treatment.

引用

页码：724 / 729

页数：6

共 50 条

[41] PLEIOTROPIC EFFECT OF 2 CELL-WALL MUTANTS ON THE ACTIVITY OF SOME CELL-WALL ENZYMES IN THE YEAST SACCHAROMYCES-CEREVISIAE
LANGE, P
MOLECULAR & GENERAL GENETICS, 1979, 173 (02): : 211 - 212
[42] Insights into the structural properties of D-serine dehydratase from Saccharomyces cerevisiae: An FT-IR spectroscopic and in silico approach
Baldassarre, Maurizio
Scire, Andrea
Fiume, Imma
Tanfani, Fabio
BIOCHIMIE, 2011, 93 (03) : 542 - 548
[43] FT-IR STUDY OF THE INTERACTION BETWEEN N,N,1,9-TETRAMETHYLGUANINE AND THE LI+ ION - COMPARISON WITH PROTONATION
PARMENTIER, J
DETAEYE, J
ZEEGERSHUYSKENS, T
BULLETIN DES SOCIETES CHIMIQUES BELGES, 1988, 97 (11-12): : 893 - 899
[44] Combining FT-IR spectroscopy and multivariate analysis for qualitative and quantitative analysis of the cell wall composition changes during apples development
Szymanska-Chargot, M.
Chylinska, M.
Kruk, B.
Zdunek, A.
CARBOHYDRATE POLYMERS, 2015, 115 : 93 - 103
[45] A procedure for the clustering of cell wall mutants in the model plant Arabidopsis based on Fourier-transform infrared (FT-IR) spectroscopy
Robin, S
Lecomte, M
Höfte, H
Mouille, G
JOURNAL OF APPLIED STATISTICS, 2003, 30 (06) : 669 - 681
[46] Live cell imaging using fourier-transform infrared (FT-IR) spectroscopy
Cheung, A.
Soh, J. Y.
Birch, B. R. P.
Cooper, A.
Lwaleed, B. A.
BRITISH JOURNAL OF SURGERY, 2011, 98 (06) : E7 - E7
[47] The effect of high external pressure on the structure and stability of MOF α-Mg3(HCOO)6 probed by in situ Raman and FT-IR spectroscopy
Mao, Haiyan
Xu, Jun
Hu, Yue
Huang, Yining
Song, Yang
JOURNAL OF MATERIALS CHEMISTRY A, 2015, 3 (22) : 11976 - 11984
[48] IMPACT OF POINT MUTATIONS AND AMINO-ACID MODIFICATIONS ON THE STRUCTURE AND STABILITY OF PEPTIDES AND PROTEINS PROBED BY FT-IR SPECTROSCOPY
FABIAN, H
NAUMANN, D
OTVOS, L
SCHULTZ, C
BACKMANN, J
SZENDREI, GI
HAHN, U
SAENGER, W
MANTSCH, HH
JOURNAL OF MOLECULAR STRUCTURE, 1995, 348 : 5 - 8
[49] Protein profile in vascular wall of atherosclerotic mice analyzed ex vivo using FT-IR spectroscopy
Wrobel, Tomasz P.
Majzner, Katarzyna
Baranska, Malgorzata
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2012, 96 : 940 - 945
[50] PRESSURE EFFECT ON HYDROGEN ISOTOPE EXCHANGE KINETICS IN CHYMOTRYPSINOGEN INVESTIGATED BY FT-IR SPECTROSCOPY
WONG, PTT
CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1991, 69 (11): : 1699 - 1704

← 1 2 3 4 5 →