Li+ effect on the cell wall of the yeast Saccharomyces cerevisiae as probed by FT-IR spectroscopy

被引：9

作者：

Zimkus, Aurelijus ^{[1
]}

Misiunas, Audrius ^{[2
]}

Chaustova, Larisa ^{[3
]}

机构：

[1] Vilnius State Univ, Dept Biochem & Biophys, LT-01513 Vilnius, Lithuania

[2] Ctr Phys Sci & Technol, Dept Organ Chem, LT-01108 Vilnius, Lithuania

[3] Vilnius State Univ, Inst Biochem, Dept Bioelectrochem & Biospectroscopy, LT-01513 Vilnius, Lithuania

来源：

CENTRAL EUROPEAN JOURNAL OF BIOLOGY | 2013年 / 8卷 / 08期

关键词：

FT-IR spectroscopy; Lithium; Saccharomyces cerevisiae; Transformation; MANNAN STRUCTURE; TRANSFORMATION; DNA; IDENTIFICATION; DYNAMICS; CANDIDA; WATER;

D O I：

10.2478/s11535-013-0186-1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effect of Li+ ions as a transformation inducing agent on the yeast cell wall has been studied. Two Saccharomyces cerevisiae strains, p63-DC5 with a native cell wall, and strain XCY42-30D(mnn1) which contains structural changes in the mannan-protein complex, were used. Fourier transform infrared (FT-IR) spectroscopy has been used for the characterization of the yeast strains and for determination of the effect of lithium cations on the cell wall. A comparison of the carbohydrate absorption band positions in the 970-1185 cm(-1) range, of Na+ and Li+ treated yeast cells has been estimated. Absorption band positions of the cell wall carbohydrates of p63-DC5 were not influenced by the studied ions. On the contrary, the treatment of XCY42-30D(mnn1) cells with Li+ ions shifted glucan band positions, implying that the cell wall structure of strain XCY42-30D(mnn1) is more sensitive to Li+ ion treatment.

引用

页码：724 / 729

页数：6

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