Comparison of PNA probe-based real-time PCR and Cobas TaqMan MTB for detection of MTBC

被引：10
|
作者：
Kim, Jae Woo [1 ]
Choi, Young Jin [2 ]
Kim, Hwi Jun [2 ]
Park, Joon Soo [3 ]
Nam, Hae Seon [4 ]
Hwangbo, Young [5 ]
Kim, Doug Uk [6 ]
Park, Kwi Sung [6 ]
机构：
[1] Soonchunhyang Univ, Coll Med, Dept Emergency Med, Cheonan 330090, South Korea
[2] Soonchunhyang Univ, Coll Med, Dept Lab Med, Cheonan 330090, South Korea
[3] Soonchunhyang Univ, Coll Med, Dept Pediat, Cheonan 330090, South Korea
[4] Soonchunhyang Univ, Coll Med, Dept Parasitol, Cheonan 330090, South Korea
[5] Soonchunhyang Univ, Coll Med, Dept Prevent Med, Cheonan 330090, South Korea
[6] Chungcheongnam Do Hlth & Environm Res Inst, Taejon 300801, South Korea
来源：
BIOCHIP JOURNAL | 2013年 / 7卷 / 02期
关键词：
Peptide nucleic acids; PCR; Mycobacterium tuberculosis complex; Tuberculosis; Real-time PCR; MYCOBACTERIUM-TUBERCULOSIS COMPLEX; DIAGNOSIS; TESTS;
D O I：
10.1007/s13206-013-7201-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We compared the sensitivities and specificities of peptide nucleic acid (PNA) probe-based real-time PCR (PNAqPCR (TM) TB/NTM) and Cobas TaqMan MTB assays for detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens. A total of 425 clinical specimens including 360 respiratory specimens and 65 non-respiratory specimens were evaluated for comparative analysis. In respiratory specimens, the sensitivity of TaqMan MTB and PNAqPCR assay for detection of MTBC was 82.9% and 91.5%, respectively. In non-respiratory specimens, the sensitivity of the TaqMan MTB and PNAqPCR assay was 23.1% and 76.9%, respectively. Overall, the sensitivity and specificity of the TaqMan MTB assay for detection of MTBC was 76.9% and 100%, respectively. The PNAqPCR assay had a sensitivity and specificity of 90% and 99.7%, respectively.
引用
收藏
页码：85 / 88
页数：4
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