Ratiometric Molecular Sensor for Monitoring Oxygen Levels in Living Cells

被引：195
|
作者：
Yoshihara, Toshitada [1 ]
Yamaguchi, Yuji [1 ]
Hosaka, Masahiro [2 ]
Takeuchi, Toshiyuki [3 ]
Tobita, Seiji [1 ]
机构：
[1] Gunma Univ, Dept Chem & Biol Chem, Grad Sch Engn, Kiryu, Gunma 3768515, Japan
[2] Akita Prefectural Univ, Dept Biotechnol, Akita 0100195, Japan
[3] Gunma Univ, Maebashi, Gunma 3718510, Japan
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 17期
基金：
日本科学技术振兴机构;
关键词：
biosensors; energy transfer; fluorescent probes; iridium; phosphorescence; ELECTRON-TRANSFER; TUMOR HYPOXIA; 2-PHOTON; MICROSCOPY; PROVIDES; DESIGN; ENERGY;
D O I：
10.1002/anie.201107557
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A ratiometric oxygen sensor (C343-Pro 4-BTP) that uses coumarin343 (blue in picture) as an oxygen-insensitive fluorophore and an iridium complex (BTP, red in picture) as an oxygen-sensitive phosphor can be used to determine the oxygen concentration in solution and in living cells, which was demonstrated by ratiometric measurements of the blue coumarin343 fluorescence and the red BTP phosphorescence in C343-Pro 4-BTP (see picture). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：4148 / 4151
页数：4
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