The role of the CD4 receptor versus HIV coreceptors in envelope-mediated apoptosis in peripheral blood mononuclear cells

被引：30

作者：

Arthos, J ^{[1
]}

Cicala, C ^{[1
]}

Selig, SM ^{[1
]}

White, AA ^{[1
]}

Ravindranath, HM ^{[1
]}

Van Ryk, D ^{[1
]}

Steenbeke, TD ^{[1
]}

Machado, E ^{[1
]}

Khazanie, P ^{[1
]}

Hanback, MS ^{[1
]}

Hanback, DB ^{[1
]}

Rabin, RL ^{[1
]}

Fauci, AS ^{[1
]}

机构：

[1] Natl Inst Hlth, NIAID, Immunoregulat Lab, Bethesda, MD 20892 USA

来源：

VIROLOGY | 2002年 / 292卷 / 01期

关键词：

CCR5; CXCR4; retrovirus; gp120; TUNEL; annexin-V; T cell;

D O I：

10.1006/viro.2001.1266

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We examined the role of CD4, CXCR4, and CCR5 in HIV envelope-mediated apoptosis by measuring the response of activated PBMCs to recombinant envelope proteins derived from CXCR4- and CCR5-utilizing viruses. Apoptosis of T cells was assessed by annexin-V staining and TdT-mediated dUTP-biotin nick-end labeling. Treatment of CCR5Delta32 homozygote PBMCs with a CCR5-specific envelope induced apoptosis in T cells, demonstrating that envelope-CD4 interactions are sufficient to induce apoptosis. However, a CXCR4-specific envelope induced higher levels of apoptosis than a CCR5-specific envelope, suggesting that envelope-mediated apoptosis can be enhanced by envelope-CXCR4 interactions. We conclude that envelope can induce apoptosis in T cells independently of the coreceptor specificity of a given envelope, or the expression profile of CXCR4 or CCR5 on a target cell. However, envelope-coreceptor interactions, and in particular, envelope-CXCR4 interactions, can contribute to this process. (C) 2002 Elsevier Science.

引用

页码：98 / 106

页数：9

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