Effects of Release-Active Antibodies to CD4 Receptor on the Level of lck-Kinase in Cultured Mononuclear Cells from Human Peripheral Blood

被引：7
|
作者：
Emel'yanova, A. G. [1 ]
Grechenko, V. V. [2 ]
Petrova, N. V. [1 ]
Shilovskii, I. P. [3 ]
Gorbunov, E. A. [2 ]
Tarasov, S. A. [2 ]
Khaitov, M. R. [3 ]
Morozov, S. G. [1 ]
Epshtein, O. I. [1 ]
机构：
[1] Res Inst Gen Pathol & Pathophysiol, Lab Physiol Act Subst, Dept Mol & Cellular Pathophysiol, Moscow, Russia
[2] Fed Med Biol Agcy Russian Federat, Res & Prod Co Mat Med Holding, Moscow, Russia
[3] Fed Med Biol Agcy Russian Federat, State Res Ctr Inst Immunol, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2017年 / 162卷 / 03期
关键词：
lck-kinase; release-activity; antibodies; CD4; Ergoferon; DOWN-REGULATION; DISSOCIATION; DEGRADATION; INFECTION; ERGOFERON; VIRUS;
D O I：
10.1007/s10517-017-3606-4
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
For evaluation of effects of release-active antibodies to CD4 on cultured lymphocytes from human peripheral blood, we measured intracellular content of lck-kinase cell-based ELISA. In cells treated with release-active antibodies to CD4, the content of intracellular lck-kinase significantly (p < 0.01) decreased in comparison with the control (purified water processed in a similar way). Phytohemagglutinin had no effect on the concentration of lck-kinase in cells. The decrease in the content of CD4-associated lck protein suggests that the preparation enhanced intracellular coupling of lck-kinase with T-cell receptor and potentiated T-cell immune response.
引用
收藏
页码：323 / 326
页数：4
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