Activity-based fingerprinting of proteases

被引：27
|
作者：
Srinivasan, R
Huang, X
Ng, SL
Yao, SQ
机构：
[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore
来源：
CHEMBIOCHEM | 2006年 / 7卷 / 01期
关键词：
activity-based profiling; enzymes; microarrays; proteases; proteomics;
D O I：
10.1002/cbic.200500340
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Not inhibited. We have successfully synthesized and tested a panel of activity-based small-molecule probes that target different classes of proteases on the basis of their enzymatic activities and substrate specificities rather than their inhibition. We have demonstrated that these probes are useful for generating unique substrate fingerprint profiles of proteases in gel-based proteomic experiments. Preliminary results indicate that they might be equally amenable for microarray-based enzyme profiling experiments. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：32 / 36
页数：5
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