Activity-based fingerprinting of proteases

被引：27
|
作者：
Srinivasan, R
Huang, X
Ng, SL
Yao, SQ
机构：
[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore
来源：
CHEMBIOCHEM | 2006年 / 7卷 / 01期
关键词：
activity-based profiling; enzymes; microarrays; proteases; proteomics;
D O I：
10.1002/cbic.200500340
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Not inhibited. We have successfully synthesized and tested a panel of activity-based small-molecule probes that target different classes of proteases on the basis of their enzymatic activities and substrate specificities rather than their inhibition. We have demonstrated that these probes are useful for generating unique substrate fingerprint profiles of proteases in gel-based proteomic experiments. Preliminary results indicate that they might be equally amenable for microarray-based enzyme profiling experiments. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：32 / 36
页数：5
相关论文
共 50 条
  • [1] Activity-based fingerprinting and inhibitor discovery of cysteine proteases in a microarray
    Uttamchandani, Mahesh
    Liu, Kai
    Panicker, Resmi C.
    Yao, Shao Q.
    CHEMICAL COMMUNICATIONS, 2007, (15) : 1518 - 1520
  • [2] Activity-Based Profiling of Proteases
    Sanman, Laura E.
    Bogyo, Matthew
    ANNUAL REVIEW OF BIOCHEMISTRY, VOL 83, 2014, 83 : 249 - 273
  • [3] Inhibitor Fingerprinting of Rhomboid Proteases by Activity-Based Protein Profiling Reveals Inhibitor Selectivity and Rhomboid Autoprocessing
    Wolf, Eliane V.
    Zeissler, Annett
    Verhelst, Steven H. L.
    ACS CHEMICAL BIOLOGY, 2015, 10 (10) : 2325 - 2333
  • [4] Activity-based proteomics of proteases in human disease
    Cravatt, B.
    JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2006, 4 : 28 - 28
  • [5] Imaging of proteases using activity-based probes
    Zmudzinski, Mikolaj
    Malon, Oliwia
    Poreba, Marcin
    Drag, Marcin
    CURRENT OPINION IN CHEMICAL BIOLOGY, 2023, 74
  • [6] Probes for activity-based profiling of plant proteases
    van der Hoorn, Renier A. L.
    Kaiser, Markus
    PHYSIOLOGIA PLANTARUM, 2012, 145 (01) : 18 - 27
  • [7] Covalent activity-based probes for imaging of serine proteases
    Skorenski, Marcin
    Ji, Shanping
    Verhelst, Steven H. L.
    BIOCHEMICAL SOCIETY TRANSACTIONS, 2024, 52 (02) : 923 - 935
  • [8] Phosphoramidates as Novel Activity-Based Probes for Serine Proteases
    Haedke, Ute R.
    Frommel, Sandra C.
    Hansen, Fabian
    Hahne, Hannes
    Kuster, Bernhard
    Bogyo, Matthew
    Verhelst, Steven H. L.
    CHEMBIOCHEM, 2014, 15 (08) : 1106 - 1110
  • [9] Peptidyl Activity-Based Probes for Imaging Serine Proteases
    Kasperkiewicz, Paulina
    FRONTIERS IN CHEMISTRY, 2021, 9
  • [10] Recent Advances in Activity-Based Protein Profiling of Proteases
    Chakrabarty, Suravi
    Kahler, Jan Pascal
    van de Plassche, Merel A. T.
    Vanhoutte, Roeland
    Verhelst, Steven H. L.
    ACTIVITY-BASED PROTEIN PROFILING, 2019, 420 : 253 - 281
12345