Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

被引：3
|
作者：
Guo, Shuai [1 ]
Nakano, Koji [1 ]
Nakajima, Hizuru [2 ]
Uchiyama, Katsumi [2 ]
Hemmi, Akihide [3 ]
Yamasaki, Yoshikazu [4 ]
Morooka, Shigeharu [4 ]
Ishimatsu, Ryoichi [1 ]
Imato, Toshihiko [1 ]
机构：
[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Fukuoka 8190395, Japan
[2] Tokyo Metropolitan Univ, Grad Sch Engn, Dept Appl Chem, Tokyo 1920397, Japan
[3] Mebius Adv Technol Ltd, Tokyo 1670042, Japan
[4] Fukuoka Univ, Fac Engn, Dept Chem Engn, Fukuoka 8140180, Japan
来源：
PURE AND APPLIED CHEMISTRY | 2012年 / 84卷 / 10期
关键词：
bioanalytical methods; binding constants; chemiluminescence immunoassay; compact disc (CD)-type microfluidic platform; enzyme chemistry; green chemistry; magnetic microbeads; optical emission spectroscopy; PERFORMANCE LIQUID-CHROMATOGRAPHY; LINKED-IMMUNOSORBENT-ASSAY; WASTE-WATER TREATMENT; ALKYLPHENOL POLYETHOXYLATES; MASS SPECTROMETRY; DEGRADATION-PRODUCTS; LINEAR ALKYLBENZENESULFONATES; WHOLE-BLOOD; NONYLPHENOL; EXTRACTION;
D O I：
10.1351/PAC-CON-11-10-10
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti-APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H2O2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC80.
引用
收藏
页码：2027 / 2043
页数：17
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