CYP4T1 - A cytochrome P450 expressed in rainbow trout (Oncorhynchus mykiss) liver

被引：13
|
作者：
Falckh, PHJ [1 ]
Wu, QK [1 ]
Ahokas, JP [1 ]
机构：
[1] RMIT UNIV,KEY CTR APPL & NUTR TOXICOL,MELBOURNE,VIC 3000,AUSTRALIA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 236卷 / 02期
关键词：
D O I：
10.1006/bbrc.1997.6957
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Total RNA isolated from a rainbow trout (Oncorhynchus mykiss) liver was subjected to RT/PCR using degenerate primers designed from homologous regions amongst cytochrome P450 CYP4 proteins. PCR amplification resulted in a single electrophoretic band which was excised, purified and sequenced directly, using cycle sequencing. The deduced protein sequence demonstrated the closest amino acid identity to rabbit CYP4B1 (54.6%) and rat CYP4B2 (55.4%). Phylogenic analysis of this sequence was found to be significantly different to any other CYP4 sequence and has been named CYP4T1. This represents the first CYP4 family member to be identified in an aquatic vertebrate. (C) 1997 Academic Press.
引用
收藏
页码：302 / 305
页数：4
相关论文
共 50 条
  • [41] CYP1A expression in liver and gills of rainbow trout (Oncorhynchus mykiss) after short-term exposure to dibenzothiophene (DBT)
    Wozny, Maciej
    Brzuzan, Pawel
    Luczynski, Michal K.
    Gora, Maciej
    Wolinska, Lidia
    Bukowski, Rafal
    Podlasz, Piotr
    CHEMOSPHERE, 2010, 79 (01) : 110 - 112
  • [42] Inhibitory effects of nicardipine to cytochrome P450 (CYP) in human liver microsomes
    Nakamura, K
    Ariyoshi, N
    Iwatsubo, T
    Fukunaga, Y
    Higuchi, S
    Itoh, K
    Shimada, N
    Nagashima, K
    Yokoi, T
    Yamamoto, K
    Horiuchi, R
    Kamataki, T
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2005, 28 (05) : 882 - 885
  • [43] Development of a monoclonal antibody for ELISA of CYP1A in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes
    Scholz, S
    Behn, I
    Honeck, H
    Hauck, C
    Braunbeck, T
    Segner, H
    BIOMARKERS, 1997, 2 (05) : 287 - 294
  • [44] Cytochrome P450 CYP1 overexpression in human tumors
    Androutsopoulos, Vasilis
    Sifakis, Stavros
    Alegakis, Athanasios
    Kiriakakis, Michalis
    Ploumidis, Achilles
    Vakonaki, Elena
    Delakas, Dimitris
    Spandidos, Demetrios
    Tsatsakis, Aristidis
    TOXICOLOGY LETTERS, 2012, 211 : S54 - S54
  • [45] CLONING AND SEQUENCING OF THE MAJOR RAINBOW-TROUT CONSTITUTIVE CYTOCHROME-P450 (CYP2K1) - IDENTIFICATION OF A NEW CYTOCHROME-P450 GENE SUBFAMILY AND ITS EXPRESSION IN MATURE RAINBOW-TROUT LIVER AND TRUNK KIDNEY
    BUHLER, DR
    YANG, YH
    DREHER, TW
    MIRANDA, CL
    WANG, JL
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1994, 312 (01) : 45 - 51
  • [46] Hepatic CYP1A induction in rainbow trout (Oncorhynchus mykiss) after exposure to benzo[a]pyrene in water
    Sandvik, M
    Horsberg, TE
    Skaare, JU
    Ingebrigtsen, K
    BIOMARKERS, 1997, 2 (03) : 175 - 180
  • [47] Identification of inhibitors of Cytochrome P450 (CYP) 1B1
    Roach, Tyler
    Kennedy, Larry
    FASEB JOURNAL, 2016, 30
  • [48] Discovery of a novel potent cytochrome P450 CYP4Z1 inhibitor
    Machalz, David
    Li, Hongjie
    Du, Wei
    Sharma, Shishir
    Liu, Sijie
    Bureik, Matthias
    Wolber, Gerhard
    EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2021, 215
  • [49] CATALYTIC ACTIVITY AND IMMUNOCHEMICAL QUANTIFICATION OF HEPATIC CYTOCHROME-P-450 IN BETA-NAPHTHOFLAVONE AND ISOSAFROL TREATED RAINBOW-TROUT (ONCORHYNCHUS-MYKISS)
    CELANDER, M
    FORLIN, L
    FISH PHYSIOLOGY AND BIOCHEMISTRY, 1991, 9 (03) : 189 - 197
  • [50] Chronic toxicity of pesticides to the mRNA expression levels of metallothioneins and cytochrome P450 1A genes in rainbow trout
    Ceyhun, Saltuk Bugrahan
    Aksakal, Ercument
    Kirim, Birsen
    Atabeyoglu, Kubra
    Erdogan, Orhan
    TOXICOLOGY AND INDUSTRIAL HEALTH, 2012, 28 (02) : 162 - 169
12345