CYP4T1 - A cytochrome P450 expressed in rainbow trout (Oncorhynchus mykiss) liver

被引：13
|
作者：
Falckh, PHJ [1 ]
Wu, QK [1 ]
Ahokas, JP [1 ]
机构：
[1] RMIT UNIV,KEY CTR APPL & NUTR TOXICOL,MELBOURNE,VIC 3000,AUSTRALIA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 236卷 / 02期
关键词：
D O I：
10.1006/bbrc.1997.6957
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Total RNA isolated from a rainbow trout (Oncorhynchus mykiss) liver was subjected to RT/PCR using degenerate primers designed from homologous regions amongst cytochrome P450 CYP4 proteins. PCR amplification resulted in a single electrophoretic band which was excised, purified and sequenced directly, using cycle sequencing. The deduced protein sequence demonstrated the closest amino acid identity to rabbit CYP4B1 (54.6%) and rat CYP4B2 (55.4%). Phylogenic analysis of this sequence was found to be significantly different to any other CYP4 sequence and has been named CYP4T1. This represents the first CYP4 family member to be identified in an aquatic vertebrate. (C) 1997 Academic Press.
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收藏
页码：302 / 305
页数：4
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