Characterization the affinity of α1A adrenoreceptor by cell membrane chromatography with frontal analysis and stoichiometric displacement model

As a bionic chromatographic method, cell membrane chromatography (CMC) has been used widely in screening active components in traditional Chinese medicine. Nevertheless, few studies have characterized the affinity between drug and receptor by CMC model. In this study, the alpha 1 adrenoreceptor (alpha(1A) AR) high expression CMC method, combined with frontal analysis and stoichiometric displacement model respectively, was established for characterizing the affinity of seven alkaloids binding to the alpha(1A) AR. The results indicate that the seven alkaloids have similar interaction strengths with tamsulosin hydrochloride (alpha(1A) AR antagonist) between them and alpha(1A) AR. In addition, electrostatic force is the main intermolecular forces between tamsulosin hydrochloride and seven alkaloids and alpha(1A) AR. The study provides a versatile approach for the characterization the affinity between drug and receptor by CMC model. (C) 2016 Elsevier B.V. All rights reserved.