Characterization the affinity of α1A adrenoreceptor by cell membrane chromatography with frontal analysis and stoichiometric displacement model

被引:14
|
作者
Wei, Fen [1 ]
Wang, Sicen [1 ]
Lv, Nan [1 ]
Bu, Yusi [1 ]
Xie, Xiaoyu [1 ]
机构
[1] Xi An Jiao Tong Univ, Sch Pharm, Hlth Sci Ctr, Xian 710061, Peoples R China
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2017年 / 1040卷
基金
中国国家自然科学基金;
关键词
Cell membrane chromatography; alpha(1) Adrenoreceptor; Frontal analysis; Stoichiometric displacement model; MASS-SPECTROMETRY; SERUM-ALBUMIN; PROTEIN; ONLINE; IMMOBILIZATION; EXPRESSION; COMPONENTS; BINDING; SYSTEM;
D O I
10.1016/j.jchromb.2016.11.002
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
As a bionic chromatographic method, cell membrane chromatography (CMC) has been used widely in screening active components in traditional Chinese medicine. Nevertheless, few studies have characterized the affinity between drug and receptor by CMC model. In this study, the alpha 1 adrenoreceptor (alpha(1A) AR) high expression CMC method, combined with frontal analysis and stoichiometric displacement model respectively, was established for characterizing the affinity of seven alkaloids binding to the alpha(1A) AR. The results indicate that the seven alkaloids have similar interaction strengths with tamsulosin hydrochloride (alpha(1A) AR antagonist) between them and alpha(1A) AR. In addition, electrostatic force is the main intermolecular forces between tamsulosin hydrochloride and seven alkaloids and alpha(1A) AR. The study provides a versatile approach for the characterization the affinity between drug and receptor by CMC model. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:273 / 281
页数:9
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