A 22 kDa polyanion inhibits carnitine-dependent fatty acid oxidation in rat liver mitochondria

The transport of activated fatty acids across the mitochondrial outer membrane has not been fully addressed. A polyanion (M-n = 22 kDa) inhibited the ADP-stimulated carnitine-dependent oxidation of both palmitoyl-CoA and palmitate plus CoA as well as mitochondrial hexokinase binding. In contrast, the oxidation of palmitoylcarnitine plus malate, as well as glutamate oxidation, was essentially unaffected. Mitochondrial carnitine palmitoyltransferase-1 was not inhibited bg the polyanion. The data suggest an additional component in carnitine-dependent mitochondrial fatty acid oxidation, possibly porin. (C) 1999 Federation of European Biochemical Societies.