Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network

被引：16

作者：

Senel, Mehmet ^{[1
]}

Coskun, Agah ^{[2
]}

Abasiyanik, M. Fatih ^{[2
]}

Bozkurt, Aayhan ^{[1
]}

机构：

[1] Fatih Univ, Fac Arts & Sci, Dept Chem, TR-34500 Istanbul, Turkey

[2] Fatih Univ, Fac Engn, Dept Genet & Bioengn, TR-34500 Istanbul, Turkey

来源：

CHEMICAL PAPERS | 2010年 / 64卷 / 01期

关键词：

urease; immobilization; proton conductivity; polymer network; SOL-GEL MATERIALS; POLYMER ELECTROLYTES; ALGINIC ACID; PROTON CONDUCTIVITY; BIOSENSORS; BLENDS; ENTRAPMENT; COPOLYMER; MEMBRANE; ENZYMES;

D O I：

10.2478/s11696-009-0103-x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study, urease was immobilized in a polymer network obtained by complexation of poly(1-vinyl imidazole) (PVI) with poly(acrylic acid) (PAA). Preparation of the polymer network was monitored by FT-IR spectroscopy. Scanning electron microscopy (SEM) revealed that enzyme immobilization had a strong effect on film morphology. Proton conductivity of the PVI/PAA network was measured via impedance spectroscopy under humidified conditions. Values of the Michaelis-Menten constant (K (M)) for immobilized urease were higher than for the free enzyme, indicating a decreased affinity of the enzyme to its substrate. The basic characteristics (pH(opt), pH(stability), T (opt), T (stability), reusability, and storage stability) of immobilized urease were determined. The results show that the PAA/PVI polymer network is suitable for enzyme immobilization.

引用

页码：1 / 7

页数：7

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