Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network

被引：16
|
作者：
Senel, Mehmet [1 ]
Coskun, Agah [2 ]
Abasiyanik, M. Fatih [2 ]
Bozkurt, Aayhan [1 ]
机构：
[1] Fatih Univ, Fac Arts & Sci, Dept Chem, TR-34500 Istanbul, Turkey
[2] Fatih Univ, Fac Engn, Dept Genet & Bioengn, TR-34500 Istanbul, Turkey
来源：
CHEMICAL PAPERS | 2010年 / 64卷 / 01期
关键词：
urease; immobilization; proton conductivity; polymer network; SOL-GEL MATERIALS; POLYMER ELECTROLYTES; ALGINIC ACID; PROTON CONDUCTIVITY; BIOSENSORS; BLENDS; ENTRAPMENT; COPOLYMER; MEMBRANE; ENZYMES;
D O I：
10.2478/s11696-009-0103-x
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In this study, urease was immobilized in a polymer network obtained by complexation of poly(1-vinyl imidazole) (PVI) with poly(acrylic acid) (PAA). Preparation of the polymer network was monitored by FT-IR spectroscopy. Scanning electron microscopy (SEM) revealed that enzyme immobilization had a strong effect on film morphology. Proton conductivity of the PVI/PAA network was measured via impedance spectroscopy under humidified conditions. Values of the Michaelis-Menten constant (K (M)) for immobilized urease were higher than for the free enzyme, indicating a decreased affinity of the enzyme to its substrate. The basic characteristics (pH(opt), pH(stability), T (opt), T (stability), reusability, and storage stability) of immobilized urease were determined. The results show that the PAA/PVI polymer network is suitable for enzyme immobilization.
引用
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页码：1 / 7
页数：7
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