An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine

被引：31

作者：

Hamidi-Asl, Ezat ^{[1
]}

Dardenne, Freddy ^{[2
]}

Blust, Ronny ^{[2
]}

De Wael, Karolien ^{[1
]}

机构：

[1] Univ Antwerp, Dept Chem, AXES Res Grp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium

[2] Univ Antwerp, Dept Biol, Sphere Res Grp, B-2020 Antwerp, Belgium

来源：

SENSORS | 2015年 / 15卷 / 04期

关键词：

PNA BIOSENSOR; DNA; OLIGONUCLEOTIDE; HYDROGELS; ELECTRODE; IMMOBILIZATION; ANTIBIOTICS; INDICATOR; SEQUENCE; RELEASE;

D O I：

10.3390/s150407605

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Because of the biocompatible properties of gelatine and the good affinity of aptamers for their targets, the combination of aptamer and gelatine type B is reported as promising for the development of biosensing devices. Here, an aptamer for chloramphenicol (CAP) is mixed with different types of gelatine and dropped on the surface of disposable gold screen printed electrodes. The signal of the CAP reduction is investigated using differential pulse voltammetry. The diagnostic performance of the sensor is described and a detection limit of 1.83 x 10(-10) M is found. The selectivity and the stability of the aptasensor are studied and compared to those of other CAP sensors described in literature.

引用

页码：7605 / 7618

页数：14

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