Computer-enhanced high-throughput genetic screens of C. elegans in a microfluidic system

被引：61
|
作者：
Crane, Matthew M. [1 ]
Chung, Kwanghun [2 ]
Lu, Hang [1 ,2 ]
机构：
[1] Georgia Inst Technol, Interdisciplinary Program Bioengn, Atlanta, GA 30332 USA
[2] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA USA
来源：
LAB ON A CHIP | 2009年 / 9卷 / 01期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
CAENORHABDITIS-ELEGANS; INTERFERENCE; RNA;
D O I：
10.1039/b813730g
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Most visual screens based on fluorescent markers are currently limited in throughput and accuracy. Here we present the first genetic screen of a mutagenized population of C. elegans in a microfluidic device. Animal handling streamlined by a microfluidic device and intuitive control software enabled the identification of novel mutants and a large screening speed.
引用
收藏
页码：38 / 40
页数：3
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