Combined scanning optical coherence and two-photon-excited fluorescence microscopy

被引：106
|
作者：
Beaurepaire, E
Moreaux, L
Amblard, F
Mertz, J
机构：
[1] Ecole Super Phys & Chim Ind, Physiol Lab, CNRS, Unite Mixte Rech 7637, F-75231 Paris 5, France
[2] Inst Curie, Lab Phys Chim Curie, CNRS, Unite Mixte Rech 168, F-75231 Paris, France
来源：
OPTICS LETTERS | 1999年 / 24卷 / 14期
关键词：
D O I：
10.1364/OL.24.000969
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We demonstrate simultaneous imaging by optical coherence microscopy (OCM) and two-photon-exerted (TPE) fluorescence microscopy. A mode-locked Ti:sapphire laser is focused and scanned in three dimensions through a fixed sample, generating both backscattered light and fluorescence light, which are independently detected. Both imaging modes provide rapid en-face imaging with submicrometer resolution. High-power delivery into the sample yields an OCM sensitivity in excess of 130 dB at 100-kHz pixel rates. Simultaneous imaging of cell nuclei with OCM and TPE is demonstrated in live drosophila embryos. (C) 1999 Optical Society of America OCIS codes: 180.6900, 170.4500, 180.5810, 180.2520, 170.3880, 190.4180.
引用
收藏
页码：969 / 971
页数：3
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