Adaptive illumination reduces photobleaching in structured illumination microscopy

被引：19
|
作者：
Chakrova, Nadya [1 ]
Canton, Alicia Soler [2 ]
Danelon, Christophe [2 ]
Stallinga, Sjoerd [1 ]
Rieger, Bernd [1 ]
机构：
[1] Delft Univ Technol, Imaging Phys Dept, Lorentzweg 1, NL-2628 CJ Delft, Netherlands
[2] Delft Univ Technol, Kavli Inst NanoSci, Dept Bionanosci, Lorentzweg 1, NL-2628 CJ Delft, Netherlands
来源：
BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 10期
关键词：
PROGRAMMABLE ARRAY MICROSCOPE; LIGHT-EXPOSURE MICROSCOPY; FLUORESCENCE MICROSCOPY; RESOLUTION LIMIT; LIVE CELLS; SUPERRESOLUTION; PHOTOTOXICITY; IMPROVEMENT; PATTERNS;
D O I：
10.1364/BOE.7.004263
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Photobleaching is a major factor limiting the observation time in fluorescence microscopy. We achieve photobleaching reduction in structured illumination microscopy (SIM) by locally adjusting the illumination intensities according to the sample. Adaptive SIM is enabled by a digital micro-mirror device (DMD), which provides a projection of the grayscale illumination patterns. We demonstrate a reduction in photobleaching by a factor of three in adaptive SIM compared to the non-adaptive SIM based on a spot grid scanning approach. Our proof-of-principle experiments show great potential for DMD-based microscopes to become a more useful tool in live-cell SIM imaging. (C) 2016 Optical Society of America
引用
收藏
页码：4263 / 4274
页数：12
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