Adaptive illumination reduces photobleaching in structured illumination microscopy

被引：19
|
作者：
Chakrova, Nadya [1 ]
Canton, Alicia Soler [2 ]
Danelon, Christophe [2 ]
Stallinga, Sjoerd [1 ]
Rieger, Bernd [1 ]
机构：
[1] Delft Univ Technol, Imaging Phys Dept, Lorentzweg 1, NL-2628 CJ Delft, Netherlands
[2] Delft Univ Technol, Kavli Inst NanoSci, Dept Bionanosci, Lorentzweg 1, NL-2628 CJ Delft, Netherlands
来源：
BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 10期
关键词：
PROGRAMMABLE ARRAY MICROSCOPE; LIGHT-EXPOSURE MICROSCOPY; FLUORESCENCE MICROSCOPY; RESOLUTION LIMIT; LIVE CELLS; SUPERRESOLUTION; PHOTOTOXICITY; IMPROVEMENT; PATTERNS;
D O I：
10.1364/BOE.7.004263
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Photobleaching is a major factor limiting the observation time in fluorescence microscopy. We achieve photobleaching reduction in structured illumination microscopy (SIM) by locally adjusting the illumination intensities according to the sample. Adaptive SIM is enabled by a digital micro-mirror device (DMD), which provides a projection of the grayscale illumination patterns. We demonstrate a reduction in photobleaching by a factor of three in adaptive SIM compared to the non-adaptive SIM based on a spot grid scanning approach. Our proof-of-principle experiments show great potential for DMD-based microscopes to become a more useful tool in live-cell SIM imaging. (C) 2016 Optical Society of America
引用
收藏
页码：4263 / 4274
页数：12
相关论文
共 50 条
  • [21] Metacrystal Structured Illumination Microscopy
    Haug, J.
    Palei, M.
    Shrout, J.
    Narimanov, E.
    Bohn, P.
    Hoffman, A. J.
    2023 IEEE PHOTONICS CONFERENCE, IPC, 2023,
  • [22] Tessellation Structured Illumination Microscopy
    Shterman, Doron
    Eyal, Ori
    Tsesses, Shai
    Bartal, Guy
    2020 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO), 2020,
  • [23] Adaptive optics for structured illumination microscopy based on deep learning
    Zheng, Yao
    Chen, Jiajia
    Wu, Chenxue
    Gong, Wei
    Si, Ke
    CYTOMETRY PART A, 2021, 99 (06) : 622 - 631
  • [24] Woofer-tweeter adaptive optical structured illumination microscopy
    Li, Qinggele
    Reinig, Marc
    Kamiyama, Daich
    Huang, Bo
    Tao, Xiaodong
    Bardales, Alex
    Kubby, Joel
    PHOTONICS RESEARCH, 2017, 5 (04) : 329 - 334
  • [25] Structured line illumination Raman microscopy
    Kozue Watanabe
    Almar F. Palonpon
    Nicholas I. Smith
    Liang-da Chiu
    Atsushi Kasai
    Hitoshi Hashimoto
    Satoshi Kawata
    Katsumasa Fujita
    Nature Communications, 6
  • [26] Multifocal structured illumination optoacoustic microscopy
    Zhenyue Chen
    Ali Özbek
    Johannes Rebling
    Quanyu Zhou
    Xosé Luís Deán-Ben
    Daniel Razansky
    Light: Science & Applications, 9
  • [27] Recent advances in structured illumination microscopy
    Ma, Ying
    Wen, Kai
    Liu, Min
    Zheng, Juanjuan
    Chu, Kaiqin
    Smith, Zachary J.
    Liu, Lixin
    Gao, Peng
    JPhys Photonics, 2021, 3 (02):
  • [28] Image processing for structured illumination microscopy
    Terui, Yuki
    2015 14TH WORKSHOP ON INFORMATION OPTICS (WIO), 2015,
  • [29] SIMPLE: Structured illumination localization microscopy
    Wieser, S.
    Reymond, L.
    Ziegler, J.
    Ruprecht, V.
    EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2019, 48 : S205 - S205
  • [30] Motionless Polarizing Structured Illumination Microscopy
    Park, Hyo Mi
    Joo, Ki-Nam
    SENSORS, 2021, 21 (08)
12345