Adaptive illumination reduces photobleaching in structured illumination microscopy

被引：19
|
作者：
Chakrova, Nadya [1 ]
Canton, Alicia Soler [2 ]
Danelon, Christophe [2 ]
Stallinga, Sjoerd [1 ]
Rieger, Bernd [1 ]
机构：
[1] Delft Univ Technol, Imaging Phys Dept, Lorentzweg 1, NL-2628 CJ Delft, Netherlands
[2] Delft Univ Technol, Kavli Inst NanoSci, Dept Bionanosci, Lorentzweg 1, NL-2628 CJ Delft, Netherlands
来源：
BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 10期
关键词：
PROGRAMMABLE ARRAY MICROSCOPE; LIGHT-EXPOSURE MICROSCOPY; FLUORESCENCE MICROSCOPY; RESOLUTION LIMIT; LIVE CELLS; SUPERRESOLUTION; PHOTOTOXICITY; IMPROVEMENT; PATTERNS;
D O I：
10.1364/BOE.7.004263
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Photobleaching is a major factor limiting the observation time in fluorescence microscopy. We achieve photobleaching reduction in structured illumination microscopy (SIM) by locally adjusting the illumination intensities according to the sample. Adaptive SIM is enabled by a digital micro-mirror device (DMD), which provides a projection of the grayscale illumination patterns. We demonstrate a reduction in photobleaching by a factor of three in adaptive SIM compared to the non-adaptive SIM based on a spot grid scanning approach. Our proof-of-principle experiments show great potential for DMD-based microscopes to become a more useful tool in live-cell SIM imaging. (C) 2016 Optical Society of America
引用
收藏
页码：4263 / 4274
页数：12
相关论文
共 50 条
  • [1] Adaptive optics for structured illumination microscopy
    Debarre, Delphine
    Botcherby, Edward J.
    Booth, Martin J.
    Wilson, Tony
    OPTICS EXPRESS, 2008, 16 (13) : 9290 - 9305
  • [2] Structured illumination microscopy
    Saxena, Manish
    Eluru, Gangadhar
    Gorthi, Sai Siva
    ADVANCES IN OPTICS AND PHOTONICS, 2015, 7 (02): : 241 - 275
  • [3] A holographic method for generating structured illumination of structured illumination microscopy
    Song, Xianlin
    HOLOGRAPHY: ADVANCES AND MODERN TRENDS VII, 2021, 11774
  • [4] Woofer–tweeter adaptive optical structured illumination microscopy
    QINGGELE LI
    MARC REINIG
    DAICH KAMIYAMA
    BO HUANG
    XIAODONG TAO
    ALEX BARDALES
    JOEL KUBBY
    Photonics Research, 2017, 5 (04) : 329 - 334
  • [5] Woofer–tweeter adaptive optical structured illumination microscopy
    QINGGELE LI
    MARC REINIG
    DAICH KAMIYAMA
    BO HUANG
    XIAODONG TAO
    ALEX BARDALES
    JOEL KUBBY
    Photonics Research , 2017, (04) : 329 - 334
  • [6] Periodic Photobleaching with Structured Illumination for Diffusion Imaging
    Cao, Ziyi
    Harmon, Dustin M.
    Yang, Ruochen
    Razumtcev, Aleksandr
    Li, Minghe
    Carlsen, Mark S.
    Geiger, Andreas C.
    Zemlyanov, Dmitry
    Sherman, Alex M.
    Takanti, Nita
    Rong, Jiayue
    Hwang, Yechan
    Taylor, Lynne S.
    Simpson, Garth J.
    ANALYTICAL CHEMISTRY, 2023, 95 (04) : 2192 - 2202
  • [7] Periodic Photobleaching with Structured Illumination for Diffusion Imaging
    Cao, Ziyi
    Harmon, Dustin M.
    Yang, Ruochen
    Razumtcev, Aleksandr
    Li, Minghe
    Carlsen, Mark S.
    Geiger, Andreas C.
    Zemlyanov, Dmitry
    Sherman, Alex M.
    Takanti, Nita
    Rong, Jiayue
    Hwang, Yechan
    Taylor, Lynne S.
    Simpson, Garth J.
    ANALYTICAL CHEMISTRY, 2023,
  • [8] Structured illumination microscopy artefacts caused by illumination scattering
    Mo, Yanquan
    Feng, Fan
    Mao, Heng
    Fan, Junchao
    Chen, Liangyi
    PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 2021, 379 (2199):
  • [9] Phase Estimation of Illumination Pattern in Structured Illumination Microscopy
    Pospisil, Jakub
    Fliegei, Karel
    Klima, Milos
    2017 27TH INTERNATIONAL CONFERENCE RADIOELEKTRONIKA (RADIOELEKTRONIKA), 2017, : 174 - 177
  • [10] Fast illumination parameter estimation of structured illumination microscopy
    Qian, Jiaming
    Cao, Yu
    Bi, Ying
    Wu, Hongjun
    Liu, Yongtao
    Chen, Qian
    Zuo, Chao
    ADVANCED OPTICAL IMAGING TECHNOLOGIES V, 2023, 12316
12345