Direct protein identification from nonspecific peptide pools by high-accuracy MS data filtering

被引：13

作者：

Thieu, Vinh An

Kirsch, Dieter

Flad, Thomas

Mueller, Claudia

Spengler, Bernhard ^{[1
]}

机构：

[1] Univ Giessen, Inst Anorgan & Analyt Chem, D-35392 Giessen, Germany

[2] Univ Tubingen, Med Klin & Poliklin, Abt 2, Sekt Transplantat Immunol & Immunhamatol, D-72072 Tubingen, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 20期

关键词：

high mass accuracy; mass spectrometry; peptide sequencing; protein structures; sequence determination;

D O I：

10.1002/anie.200503787

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Validate your results! High-accuracy mass spectrometric analysis and composition-based de novo sequencing (CBS) combined with database(DB)-assisted protein identification allows for the rapid production of validated analytical results in proteome research. A simplified sample preparation scheme is sufficient to provide for reliable identification of proteins even when following nonspecific and unknown degradation processes. (Figure Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3317 / 3319

页数：3

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