Direct protein identification from nonspecific peptide pools by high-accuracy MS data filtering

被引：13
|
作者：
Thieu, Vinh An
Kirsch, Dieter
Flad, Thomas
Mueller, Claudia
Spengler, Bernhard [1 ]
机构：
[1] Univ Giessen, Inst Anorgan & Analyt Chem, D-35392 Giessen, Germany
[2] Univ Tubingen, Med Klin & Poliklin, Abt 2, Sekt Transplantat Immunol & Immunhamatol, D-72072 Tubingen, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 20期
关键词：
high mass accuracy; mass spectrometry; peptide sequencing; protein structures; sequence determination;
D O I：
10.1002/anie.200503787
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Validate your results! High-accuracy mass spectrometric analysis and composition-based de novo sequencing (CBS) combined with database(DB)-assisted protein identification allows for the rapid production of validated analytical results in proteome research. A simplified sample preparation scheme is sufficient to provide for reliable identification of proteins even when following nonspecific and unknown degradation processes. (Figure Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：3317 / 3319
页数：3
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