Molecular characterization of a cell wall-associated β(1-3)endoglucanase of Aspergillus fumigatus

被引：0
|
作者：
Mouyna, I
Sarfati, J
Recco, P
Fontaine, T
Henrissat, B
Latge, JP
机构：
[1] Inst Pasteur, Unite Aspergillus, F-75724 Paris 15, France
[2] CHU Rangueil, Serv Parasitol Mycol, F-31054 Toulouse, France
[3] CNRS, IFRI, Architecture & Fonct Macromol Biol, Marseille, France
来源：
MEDICAL MYCOLOGY | 2002年 / 40卷 / 05期
关键词：
Aspergillus fumigatus; beta(1-3)endoglucanase; cell wall; ENGL1;
D O I：
10.1080/714031140
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A 74 kDa beta(1-3)endoglucanase of Aspergillus fumigatus was recently isolated from a cell wall autolysate and biochemically characterized. In this study, we report the cloning and the disruption of the ENGL1 gene encoding this beta(1-3)endoglucanase. ENGL1 contains an open reading frame of 2181 bp encoding a polypeptide of 727 amino acids. Sequence analysis showed that ENGL1 is the first characterized member of a new family of beta(1-3)glucanases. Disruption of ENGL1, however, did not lead to a phenotype distinct from the parental strain, indicating that this cell wall-associated beta(1-3)endoglucanase does not play an essential role in constitutive cell growth.
引用
收藏
页码：455 / 464
页数：10
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