Simplified Two-Step Purification of Hyaluronidase from Buthus martensii Manchurian Scorpion Venom

被引：0

作者：

Wali, A. ^{[1
]}

Yang, Z. ^{[1
,2
]}

Arken, A. ^{[1
,2
]}

Ali, Y. ^{[1
,2
]}

Wang, Y. H. ^{[1
,2
]}

Kelaimu, R. ^{[1
,2
]}

Mavlonov, G. T. ^{[3
]}

Yili, A. ^{[1
]}

Aisa, H. A. ^{[1
]}

机构：

[1] Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plants, Key Lab Chem Plant Resources Arid Reg, South Beijing Rd 40-1, Urumqi, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100039, Peoples R China

[3] Acad Sci Uzbek, A S Sadykov Inst Bioorgan Chem, Tashkent 100125, Uzbekistan

来源：

CHEMISTRY OF NATURAL COMPOUNDS | 2023年 / 59卷 / 02期

关键词：

Buthus martensii scorpion venom; hyaluronidase; cation-exchange chromatography; centrifugal ultrafiltration;

D O I：

10.1007/s10600-023-03992-y

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Whole venom of Buthus martensii Karsch Manchurian scorpion was collected by electrostimulation and was fractionated by cation-exchange chromatography over Toyopearl CM650-M followed by ultrafiltration through an Amicon Ultra-15 membrane with a cut-off threshold of 10 kDa to isolate hyaluronidase. The specific activity of the purified hyaluronidase was 16,000 TRU/mg. The degree of purification calculated from the increase of specific activity was 71x; the yield, 75.6%. The electrophoretic and chromatographic purities were 88.4% and 82.5%, respectively.

引用

页码：354 / 356

页数：3

共 50 条

[41] Purification and primary structure of low molecular mass peptides from scorpion (Buthus sindicus) venom
Ali, SA
Stoeva, S
Schütz, J
Kayed, R
Abassi, A
Zaidi, ZH
Voelter, W
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 1998, 121 (04): : 323 - 332
[42] Purification, characterization, and bioactivity of a new analgesic-antitumor peptide from Chinese scorpion Buthus martensii Karsch
Shao, Jian-Hua
Cui, Yong
Zhao, Ming-Yi
Wu, Chun-Fu
Liu, Yan-Feng
Zhang, Jing-Hai
PEPTIDES, 2014, 53 : 89 - 96
[43] A series of bioactivity-variant neurotoxins from scorpion Buthus martensii Karsch:: purification, crystallization and crystallographic analysis
Li, HM
Zhao, T
Jin, L
Wang, M
Zhang, Y
Wang, DC
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1999, 55 : 341 - 344
[44] Proteomic analysis of the venom from the scorpion Mesobuthus martensii
Xu, Xiaobo
Duan, Zhigui
Di, Zhiyong
He, Yawen
Li, Jianglin
Li, Zhongjie
Xie, Chunliang
Zeng, Xiongzhi
Cao, Zhijian
Wu, Yingliang
Liang, Songping
Li, Wenxin
JOURNAL OF PROTEOMICS, 2014, 106 : 162 - 180
[45] Study of Analgesic Effect of Scorpion Venom(Buthus Martensii Karshi Liaoning)on Rat Hippocampal Slices in vitro
针刺研究, 1997, (Z1) : 71 - 71
[46] Purification and N-terminal sequence of a serine proteinase-like protein (BMK-CBP) from the venom of the Chinese scorpion (Buthus martensii Karsch)
Gao, Rong
Zhang, Yong
Gopalakrishnakone, Ponnampalam
TOXICON, 2008, 52 (02) : 348 - 353
[47] Purification and characterization of an analgesic peptide from Buthus martensii Karsch
Shao, Jianhua
Kang, Ning
Liu, Yanfeng
Song, Shuang
Wu, Chunfu
Zhang, Jinghai
BIOMEDICAL CHROMATOGRAPHY, 2007, 21 (12) : 1266 - 1271
[48] Purification, crystallization and initial structural solution of a new α-like toxin with cardiac toxicity from scorpion Buthus martensii Karsch
Guan, RJ
He, XL
Wang, M
Ye-Xiang
Li, GP
Wang, DC
PROTEIN AND PEPTIDE LETTERS, 2002, 9 (05): : 441 - 449
[49] A new insect neurotoxin AngP1 with analgesic effect from the scorpion Buthus martensii Karsch:: purification and characterization
Guan, RJ
Wang, M
Wang, D
Wang, DC
JOURNAL OF PEPTIDE RESEARCH, 2001, 58 (01): : 27 - 35
[50] PURIFICATION, CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF A NEUROTOXIN WITH MEDIUM TOXICITY FROM CHINESE SCORPION (BUTHUS-MARTENSII KARSCH)
LI, HM
WANG, M
LIU, YS
ZHAO, T
JIN, L
WANG, DC
ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 1995, 27 (02): : 145 - 151

← 1 2 3 4 5 →