Simplified Two-Step Purification of Hyaluronidase from Buthus martensii Manchurian Scorpion Venom

被引：0
|
作者：
Wali, A. [1 ]
Yang, Z. [1 ,2 ]
Arken, A. [1 ,2 ]
Ali, Y. [1 ,2 ]
Wang, Y. H. [1 ,2 ]
Kelaimu, R. [1 ,2 ]
Mavlonov, G. T. [3 ]
Yili, A. [1 ]
Aisa, H. A. [1 ]
机构：
[1] Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plants, Key Lab Chem Plant Resources Arid Reg, South Beijing Rd 40-1, Urumqi, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
[3] Acad Sci Uzbek, A S Sadykov Inst Bioorgan Chem, Tashkent 100125, Uzbekistan
来源：
CHEMISTRY OF NATURAL COMPOUNDS | 2023年 / 59卷 / 02期
关键词：
Buthus martensii scorpion venom; hyaluronidase; cation-exchange chromatography; centrifugal ultrafiltration;
D O I：
10.1007/s10600-023-03992-y
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Whole venom of Buthus martensii Karsch Manchurian scorpion was collected by electrostimulation and was fractionated by cation-exchange chromatography over Toyopearl CM650-M followed by ultrafiltration through an Amicon Ultra-15 membrane with a cut-off threshold of 10 kDa to isolate hyaluronidase. The specific activity of the purified hyaluronidase was 16,000 TRU/mg. The degree of purification calculated from the increase of specific activity was 71x; the yield, 75.6%. The electrophoretic and chromatographic purities were 88.4% and 82.5%, respectively.
引用
收藏
页码：354 / 356
页数：3
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