Characterization of the Keratinolytic Activity of Three Streptomyces Strains and Impact of Their Co-Cultivation on This Activity

被引:3
|
作者
Martin-Gonzalez, Diego [1 ,2 ]
Bordel, Sergio [1 ,2 ]
Santos-Beneit, Fernando [1 ,2 ,3 ]
机构
[1] Inst Sustainable Proc, Dr Mergelina S-N, Valladolid 47011, Spain
[2] Univ Valladolid, Sch Ind Engn, Dept Chem Engn & Environm Technol, Dr Mergelina S-N, Valladolid 47011, Spain
[3] Univ Oviedo, Med Sch, Dept Funct Biol, Ave Julian Claveria 6, Oviedo 33006, Spain
关键词
Streptomyces; undecylprodigiosin; Staphylococcus aureus; keratinase; antibiotic; feathers; biofertilizers; co-culture; PHO REGULON; FEATHER; GENES;
D O I
10.3390/microorganisms11051109
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In this study, we describe the characterization of three efficient chicken feather-degrading Streptomyces bacteria isolated from honeybee samples and assess the impact of their co-cultivation on this activity and antistaphylococcal activity. Streptomyces griseoaurantiacus AD2 was the strain showing the highest keratinolytic activity (4000 U x mL(-1)), followed by Streptomyces albidoflavus AN1 and Streptomyces drozdowiczii AD1, which both generated approximately 3000 U x mL(-1). Moreover, a consortium constituted of these three strains was able to use chicken feathers as its sole nutrient source and growth in such conditions led to a significant increase in antibiotic production. S. griseoaurantiacus AD2 was the only strain that exhibited weak antimicrobial activity against Staphylococcus aureus. UPLC analyses revealed that a significant number of peaks detected in the extracts of co-cultures of the three strains were missing in the extracts of individual cultures. In addition, the production of specialized metabolites, such as undecylprodigiosin and manumycin A, was clearly enhanced in co-culture conditions, in agreement with the results of the antimicrobial bioassays against S. aureus. Our results revealed the benefits of co-cultivation of these bacterial species in terms of metabolic wealth and antibiotic production. Our work could thus contribute to the development of novel microbial-based strategies to valorize keratin waste.
引用
收藏
页数:10
相关论文
共 50 条
  • [31] Keratinolytic activity of Streptomyces sp SK1-02:: a new isolated strain
    Letourneau, F
    Soussotte, V
    Bressollier, P
    Branland, P
    Verneuil, B
    LETTERS IN APPLIED MICROBIOLOGY, 1998, 26 (01) : 77 - 80
  • [32] Co-cultivation of Streptomyces californicus and Stachybotrys chartarum stimulates the production of cytostatic compound(s) with immunotoxic properties
    Penttinen, Piia
    Pelkonen, Jukka
    Huttunen, Kati
    Hirvonen, Maija-Riitta
    TOXICOLOGY AND APPLIED PHARMACOLOGY, 2006, 217 (03) : 342 - 351
  • [33] Enhanced production of undecylprodigiosin in Streptomyces coelicolor by co-cultivation with the corallopyronin A-producing myxobacterium, Corallococcus coralloides
    Till F. Schäberle
    Annika Orland
    Gabriele M. König
    Biotechnology Letters, 2014, 36 : 641 - 648
  • [34] Effect of co-cultivation of white and brown rot species on basidiome production, lignocelluloytic enzyme activity and dye decolourisation
    Barbelli-Lopez, Maria Soledad
    Peralta, Maria Patricia
    Levin, Laura
    Lechner, Bernardo Ernesto
    BIORESOURCE TECHNOLOGY, 2024, 395
  • [35] Enhanced production of undecylprodigiosin in Streptomyces coelicolor by co-cultivation with the corallopyronin A-producing myxobacterium, Corallococcus coralloides
    Schaeberle, Till F.
    Orland, Annika
    Koenig, Gabriele M.
    BIOTECHNOLOGY LETTERS, 2014, 36 (03) : 641 - 648
  • [36] Enhancement of ligninolytic enzymes production and decolourising activity in Leptosphaerulina sp by co-cultivation with Trichoderma viride and Aspergillus terreus
    Copete-Pertuz, Ledys S.
    Alandete-Novoa, Felipe
    Placido, Jersson
    Correa-Londono, Guillermo A.
    Mora-Martinez, Amanda L.
    SCIENCE OF THE TOTAL ENVIRONMENT, 2019, 646 : 1536 - 1545
  • [37] Successful Isolation of Diverse Verrucomicrobiota Strains through the Duckweed- Microbes Co-cultivation Method
    Tanaka, Yasuhiro
    Tozawa, Erina
    Iwashita, Tomoki
    Morishita, Yosuke
    Tamaki, Hideyuki
    Toyama, Tadashi
    Morikawa, Masaaki
    Kamagata, Toichi
    Mori, Kazuhiro
    MICROBES AND ENVIRONMENTS, 2024, 39 (03)
  • [38] Co-cultivation of Sorangium cellulosum strains affects cellular growth and biosynthesis of secondary metabolite epothilones
    Li, Peng-fei
    Li, Shu-guang
    Li, Zhi-feng
    Zhao, Lin
    Wang, Ting
    Pan, Hong-wei
    Liu, Hong
    Wu, Zhi-hong
    Li, Yue-zhong
    FEMS MICROBIOLOGY ECOLOGY, 2013, 85 (02) : 358 - 368
  • [39] Keratinolytic activity of pigmenting and non-pigmenting soils strains of Trichophyton ajelloi
    Bohacz, Justyna
    Mozejko, Michal
    INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2024, 186
  • [40] Beneficial impact of primary RPE cells in a porcine organotypic co-cultivation model
    Joachim, Stephanie C.
    Wagner, Natalie
    Safaei, Armin
    Hurst, Jose
    Vogt, Pia
    Gammel, Maurice R.
    Dick, H. Bukhard
    Schnichels, Sven
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2022, 63 (07)