Keratinolytic activity of Streptomyces sp SK1-02:: a new isolated strain

被引:0
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作者
Letourneau, F [1 ]
Soussotte, V [1 ]
Bressollier, P [1 ]
Branland, P [1 ]
Verneuil, B [1 ]
机构
[1] IUT, Dept Appl Biol, Lab GEnie Enzymat & Biovalorisat, F-87065 Limoges, France
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中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A Streptomyces sp. producing a high keratinolytic activity when cultured on feather meal medium was isolated from a naturally degraded feather. Maximal keratin degradation using supernatant fluid obtained from batch culture of this organism was observed at 70 degrees C and pH 10. Keratinolytic activity was only partially inhibited by EDTA or PMSF, suggesting that the overall keratinolytic activity was supported by different proteases. Comparisons between proteolytic activities derived from this new strain (S.K1-02) and commercial proteases indicated that S.K1-02 could be a useful biotechnological tool in valorization of keratin-containing wastes, or in the depilation process in the leather industry.
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页码:77 / 80
页数:4
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