Oxidative Stress and DNA Damage in Pagrus major by the Dinoflagellate Karenia mikimotoi

被引:3
|
作者
Shin, Yun Kyung [1 ]
Seo, Do Yeon [2 ,3 ]
Eom, Hye-Jin [2 ]
Park, Mira [4 ]
Lee, Minji [5 ]
Choi, Young-Eun [2 ,6 ]
Han, Young-Seok [7 ]
Rhee, Jae-Sung [2 ,4 ,8 ]
Kim, Youn-Jung [2 ,4 ,8 ]
机构
[1] Natl Inst Fisheries Sci, Busan 46083, South Korea
[2] Incheon Natl Univ, Dept Marine Sci, Coll Nat Sci, Incheon 22012, South Korea
[3] Natl Inst Environm Res, Environm Hlth Res Dept, Risk Assessment Div, Incheon 22689, South Korea
[4] Incheon Natl Univ, Res Inst Basic Sci, Incheon 22012, South Korea
[5] South Sea Fisheries Res Inst, Natl Inst Fisheries Sci NIFS, Yeosu 59780, South Korea
[6] Eco Sustainable Solut Ctr Korea Conform Labs, Dept Neurol, Incheon 40684, South Korea
[7] Neo Environm Business Co, Bucheon 14523, South Korea
[8] Yellow Sea Res Inst, Incheon 22012, South Korea
基金
新加坡国家研究基金会;
关键词
Karenia mikimotoi; Pagrus major; oxidative stress; DNA damage; RED-TIDE DINOFLAGELLATE; HARMFUL ALGAL BLOOMS; MOLECULAR ECOLOGICAL RESPONSES; ANTIOXIDANT RESPONSES; GYRODINIUM-AUREOLUM; LIPID-PEROXIDATION; HYDROGEN-PEROXIDE; COASTAL WATERS; OXYGEN LEVELS; SEA;
D O I
10.3390/toxins15100620
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Karenia mikimotoi is a common species of red tide dinoflagellate that causes the mass mortality of marine fauna in coastal waters of Republic of Korea. Despite continuous studies on the ecophysiology and toxicity of K. mikimotoi, the underlying molecular mechanisms remain poorly understood. Red sea bream, Pagrus major, is a high-value aquaculture fish species, and the coastal aquaculture industry of red sea bream has been increasingly affected by red tides. To investigate the potential oxidative effects of K. mikimotoi on P. major and the molecular mechanisms involved, we exposed the fish to varying concentrations of K. mikimotoi and evaluated its toxicity. Our results showed that exposure to K. mikimotoi led to an accumulation of reactive oxygen species (ROS) and oxidative DNA damage in the gill tissue of P. major. Furthermore, we found that K. mikimotoi induced the activation of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, in the gill tissue of P. major, with a significant increase in activity at concentrations above 5000 cells/mL. However, the activity of glutathione S-transferase did not significantly increase at the equivalent concentration. Our study confirms that oxidative stress and DNA damage is induced by acute exposure to K. mikimotoi, as it produces ROS and hypoxic conditions in P. major. In addition, it was confirmed that gill and blood samples can be used as biomarkers to detect the degree of oxidative stress in fish. These findings have important implications for the aquaculture of red sea bream, particularly in the face of red tide disasters.
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页数:16
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