Oxidative Stress and DNA Damage in Pagrus major by the Dinoflagellate Karenia mikimotoi

被引:3
|
作者
Shin, Yun Kyung [1 ]
Seo, Do Yeon [2 ,3 ]
Eom, Hye-Jin [2 ]
Park, Mira [4 ]
Lee, Minji [5 ]
Choi, Young-Eun [2 ,6 ]
Han, Young-Seok [7 ]
Rhee, Jae-Sung [2 ,4 ,8 ]
Kim, Youn-Jung [2 ,4 ,8 ]
机构
[1] Natl Inst Fisheries Sci, Busan 46083, South Korea
[2] Incheon Natl Univ, Dept Marine Sci, Coll Nat Sci, Incheon 22012, South Korea
[3] Natl Inst Environm Res, Environm Hlth Res Dept, Risk Assessment Div, Incheon 22689, South Korea
[4] Incheon Natl Univ, Res Inst Basic Sci, Incheon 22012, South Korea
[5] South Sea Fisheries Res Inst, Natl Inst Fisheries Sci NIFS, Yeosu 59780, South Korea
[6] Eco Sustainable Solut Ctr Korea Conform Labs, Dept Neurol, Incheon 40684, South Korea
[7] Neo Environm Business Co, Bucheon 14523, South Korea
[8] Yellow Sea Res Inst, Incheon 22012, South Korea
基金
新加坡国家研究基金会;
关键词
Karenia mikimotoi; Pagrus major; oxidative stress; DNA damage; RED-TIDE DINOFLAGELLATE; HARMFUL ALGAL BLOOMS; MOLECULAR ECOLOGICAL RESPONSES; ANTIOXIDANT RESPONSES; GYRODINIUM-AUREOLUM; LIPID-PEROXIDATION; HYDROGEN-PEROXIDE; COASTAL WATERS; OXYGEN LEVELS; SEA;
D O I
10.3390/toxins15100620
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Karenia mikimotoi is a common species of red tide dinoflagellate that causes the mass mortality of marine fauna in coastal waters of Republic of Korea. Despite continuous studies on the ecophysiology and toxicity of K. mikimotoi, the underlying molecular mechanisms remain poorly understood. Red sea bream, Pagrus major, is a high-value aquaculture fish species, and the coastal aquaculture industry of red sea bream has been increasingly affected by red tides. To investigate the potential oxidative effects of K. mikimotoi on P. major and the molecular mechanisms involved, we exposed the fish to varying concentrations of K. mikimotoi and evaluated its toxicity. Our results showed that exposure to K. mikimotoi led to an accumulation of reactive oxygen species (ROS) and oxidative DNA damage in the gill tissue of P. major. Furthermore, we found that K. mikimotoi induced the activation of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, in the gill tissue of P. major, with a significant increase in activity at concentrations above 5000 cells/mL. However, the activity of glutathione S-transferase did not significantly increase at the equivalent concentration. Our study confirms that oxidative stress and DNA damage is induced by acute exposure to K. mikimotoi, as it produces ROS and hypoxic conditions in P. major. In addition, it was confirmed that gill and blood samples can be used as biomarkers to detect the degree of oxidative stress in fish. These findings have important implications for the aquaculture of red sea bream, particularly in the face of red tide disasters.
引用
收藏
页数:16
相关论文
共 50 条
  • [21] Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi
    Richard G. Dorrell
    George A. Hinksman
    Christopher J. Howe
    Plant Molecular Biology, 2016, 90 : 233 - 247
  • [22] Microplastic-induced apoptosis and metabolism responses in marine Dinoflagellate, Karenia mikimotoi
    Zhao, Ting
    Tan, Liju
    Han, Xiaotian
    Wang, Xutao
    Zhang, Yafeng
    Ma, Xiaofeng
    Lin, Kun
    Wang, Rui
    Ni, Ziqi
    Wang, Jiayin
    Wang, Jiangtao
    SCIENCE OF THE TOTAL ENVIRONMENT, 2022, 804
  • [23] Effects of the dinoflagellate Karenia mikimotoi on larval settlement of Pacific oyster Crassostrea gigas
    Mizuno, Ken-ichiro
    Wakano, Makoto
    Takatsuji, Hideyuki
    Nagai, Takahiro
    NIPPON SUISAN GAKKAISHI, 2015, 81 (05) : 811 - 816
  • [24] Effects of ocean acidification and phosphate limitation on physiology and toxicity of the dinoflagellate Karenia mikimotoi
    Wang, Hong
    Niu, Xiaoqin
    Feng, Xinqian
    Goncalves, Rodrigo J.
    Guan, Wanchun
    HARMFUL ALGAE, 2019, 87
  • [25] Toxic effects of the dinoflagellate Karenia mikimotoi on zebrafish (Danio rerio) larval behavior
    Niu, Xiaoqin
    Xu, Shengnan
    Yang, Qiongying
    Xu, Xuelian
    Zheng, Miaomiao
    Li, Xi
    Guan, Wanchun
    HARMFUL ALGAE, 2021, 103
  • [26] Detrimental impacts of the dinoflagellate Karenia mikimotoi in Fujian coastal waters on typical marine organisms
    Li, Xiaodong
    Yan, Tian
    Lin, Jianing
    Yu, Rencheng
    Zhou, Mingjiang
    HARMFUL ALGAE, 2017, 61 : 1 - 12
  • [27] Detrimental effect of the harmful dinoflagellate Karenia mikimotoi on Japanese common squid Todarodes pacificus
    Natsuike, Masafumi
    Yamamoto, Jun
    Konishi, Tetsuya
    Kimura, Shunsuke
    Kitagawa, Masahiko
    Itaya, Kazuhiko
    FISHERIES SCIENCE, 2024, 90 (06) : 925 - 930
  • [28] RNA Sequencing Revealed Numerous Polyketide Synthase Genes in the Harmful Dinoflagellate Karenia mikimotoi
    Kimura, Kei
    Okuda, Shujiro
    Nakayama, Kei
    Shikata, Tomoyuki
    Takahashi, Fumio
    Yamaguchi, Haruo
    Skamoto, Setsuko
    Yamaguchi, Mineo
    Tomaru, Yuji
    PLOS ONE, 2015, 10 (11):
  • [29] Efficient modulation of cellular phosphorus components in response to phosphorus deficiency in the dinoflagellate Karenia mikimotoi
    Huang, Xue-Ling
    Zhuang, Yan-Qing
    Xiong, Yue-Yue
    Li, Da-Wei
    Ou, Lin-Jian
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2023, 89 (11)
  • [30] Inhibition to crucial enzymes in the lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis
    Li, Xiao-dong
    Yan, Tian
    Zhang, Qing-chun
    Yu, Ren-cheng
    Zhou, Ming-jiang
    MARINE ENVIRONMENTAL RESEARCH, 2020, 157 (157)