Improving optical sectioning with spinning disk structured illumination microscopy

被引：2
|
作者：
Paul, Tristan C. [1 ,2 ]
Hagen, Guy [2 ]
机构：
[1] Univ Colorado, Dept Phys & Energy Sci, 1420 Austin Bluffs Pkwy, Colorado Springs, CO 80918 USA
[2] UCCS BioFrontiers Ctr, 1420 Austin Bluffs Pkwy, Colorado Springs, CO 80918 USA
来源：
OPTICS EXPRESS | 2023年 / 31卷 / 23期
基金：
美国国家卫生研究院;
关键词：
CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; RESOLUTION; LIGHT;
D O I：
10.1364/OE.499277
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
A new fluorescence microscopy technique for optical sectioning was investigated. This technique combined Spinning Disk microscopy (SD) with Structured Illumination Microscopy (SIM), resulting in more background removal than either method. Spinning Disk Structured Illumination Microscopy (SD-SIM) resulted in higher signal-to-background ratios. The method detected and quantified a dendritic spine neck that was impossible to detect with either SIM or SD alone.
引用
收藏
页码：38831 / 38839
页数：9
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