Improving optical sectioning with spinning disk structured illumination microscopy

被引：2
|
作者：
Paul, Tristan C. [1 ,2 ]
Hagen, Guy [2 ]
机构：
[1] Univ Colorado, Dept Phys & Energy Sci, 1420 Austin Bluffs Pkwy, Colorado Springs, CO 80918 USA
[2] UCCS BioFrontiers Ctr, 1420 Austin Bluffs Pkwy, Colorado Springs, CO 80918 USA
来源：
OPTICS EXPRESS | 2023年 / 31卷 / 23期
基金：
美国国家卫生研究院;
关键词：
CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; RESOLUTION; LIGHT;
D O I：
10.1364/OE.499277
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
A new fluorescence microscopy technique for optical sectioning was investigated. This technique combined Spinning Disk microscopy (SD) with Structured Illumination Microscopy (SIM), resulting in more background removal than either method. Spinning Disk Structured Illumination Microscopy (SD-SIM) resulted in higher signal-to-background ratios. The method detected and quantified a dendritic spine neck that was impossible to detect with either SIM or SD alone.
引用
收藏
页码：38831 / 38839
页数：9
相关论文
共 50 条
  • [31] Multi-view object topography measurement with optical sectioning structured illumination microscopy
    Ren, Feifei
    Wang, Zhaojun
    Qian, Jia
    Liang, Yansheng
    Dang, Shipei
    Cai, Yanan
    Bianco, Piero R.
    Yao, Baoli
    Lei, Ming
    APPLIED OPTICS, 2019, 58 (23) : 6288 - 6294
  • [32] Optical-sectioning microscopy by patterned illumination
    Saavedra, G.
    Martinez-Corral, M.
    Sanchez-Ortiga, E.
    Doblas, A.
    2009 INTERNATIONAL WORKSHOP ON INFORMATION OPTICS, 2010, 206
  • [33] Optical sectioning by selective illumination feedback microscopy
    Krzewina, Leo G.
    Kim, Myung K.
    JOURNAL OF MODERN OPTICS, 2007, 54 (12) : 1819 - 1826
  • [34] Background Noise Suppression of Optical Sectioning Structured Illumination Microscopy via Fourier Domain Reconstruction
    Dang, Shipei
    Qian, Jia
    Peng, Tong
    Bai, Chen
    Min, Junwei
    Wang, Haixia
    Yao, Baoli
    Dan, Dan
    FRONTIERS IN PHYSICS, 2022, 10
  • [35] Double-Exposure Optical Sectioning Structured Illumination Microscopy Based on Hilbert Transform Reconstruction
    Zhou, Xing
    Lei, Ming
    Dan, Dan
    Yao, Baoli
    Qian, Jia
    Yan, Shaohui
    Yang, Yanlong
    Min, Junwei
    Peng, Tong
    Ye, Tong
    Chen, Guangde
    PLOS ONE, 2015, 10 (03):
  • [36] Adaptive structured illumination optical-sectioning microscopy based on the prior knowledge of sample structure
    Li, Dongzhe
    Zhou, Weishuai
    Qiu, Zhenmin
    Peng, Junzheng
    Zhong, Jingang
    OPTICS AND LASERS IN ENGINEERING, 2024, 172
  • [37] Optimized approaches for optical sectioning and resolution enhancement in 2D structured illumination microscopy
    O'Holleran, Kevin
    Shaw, Michael
    BIOMEDICAL OPTICS EXPRESS, 2014, 5 (08): : 2580 - 2590
  • [38] Background Noise Suppression of Optical Sectioning Structured Illumination Microscopy via Fourier Domain Reconstruction
    Dang, Shipei
    Qian, Jia
    Peng, Tong
    Bai, Chen
    Min, Junwei
    Wang, Haixia
    Yao, Baoli
    Dan, Dan
    Frontiers in Physics, 2022, 10
  • [39] Enhanced multifocal structured illumination microscopy with desired optical sectioning capability and lateral resolution improvement
    Zhang, Chenshuang
    Zhang, Wei
    Yu, Bin
    Lin, Danying
    Qu, Junle
    OPTICS EXPRESS, 2020, 28 (25): : 37946 - 37957
  • [40] Incoherent structured illumination improves optical sectioning and contrast in multiphoton super-resolution microscopy
    Winter, Peter W.
    Chandris, Panagiotis
    Fischer, Robert S.
    Wu, Yicong
    Waterman, Clare M.
    Shroff, Hari
    OPTICS EXPRESS, 2015, 23 (04): : 5327 - 5334
12345