Enriching adenosine by thymine-rich DNA oligomers

被引：1

作者：

Liu, Mingchun ^{[1
]}

Chen, Huaiqing ^{[2
]}

Huang, Yuhan ^{[1
]}

Liu, Jian ^{[1
]}

Chen, Qianfeng ^{[1
]}

Zuo, Hua ^{[1
]}

Fang, Liang ^{[3
,4
]}

Mao, Chengde ^{[1
,5
]}

机构：

[1] Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China

[2] Southwest Univ, Biol Sci Res Ctr, State Key Lab Silkworm Genome Biol, Chongqing 400715, Peoples R China

[3] Ninth Chongqing Peoples Hosp, Dept Oncol, Chongqing 400700, Peoples R China

[4] Second Affiliated Hosp Chongqing Med Univ, Dept Hematol, Chongqing 400010, Peoples R China

[5] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

ANALYST | 2023年 / 148卷 / 08期

基金：

美国国家科学基金会;

关键词：

ENRICHMENT; NUCLEOSIDES; SEPARATION; RECEPTORS; CAPTURE; SILICA; ACID;

D O I：

10.1039/d3an00297g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Adenosine levels are important in various physiological and pathological activities, but detecting them is difficult because of interference from a complex matrix. This study designed a series of DNA oligomers rich in thymine to enrich adenosine. Their binding affinity (K-d range: 1.25-5.0 mM) to adenosine varied based on the DNA secondary structures, with a clamped hairpin structure showing the highest binding affinity. Compared to other designs, this clamped DNA hairpin underwent the least conformational change during adenosine binding. These DNAs also suppressed the precipitation of supersaturated adenine. Taken together, these results suggest that thymine-rich DNAs could be used to enrich and separate adenosine.

引用

页码：1858 / 1866

页数：9

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