Enriching adenosine by thymine-rich DNA oligomers

被引：1

作者：

Liu, Mingchun ^{[1
]}

Chen, Huaiqing ^{[2
]}

Huang, Yuhan ^{[1
]}

Liu, Jian ^{[1
]}

Chen, Qianfeng ^{[1
]}

Zuo, Hua ^{[1
]}

Fang, Liang ^{[3
,4
]}

Mao, Chengde ^{[1
,5
]}

机构：

[1] Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China

[2] Southwest Univ, Biol Sci Res Ctr, State Key Lab Silkworm Genome Biol, Chongqing 400715, Peoples R China

[3] Ninth Chongqing Peoples Hosp, Dept Oncol, Chongqing 400700, Peoples R China

[4] Second Affiliated Hosp Chongqing Med Univ, Dept Hematol, Chongqing 400010, Peoples R China

[5] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

ANALYST | 2023年 / 148卷 / 08期

基金：

美国国家科学基金会;

关键词：

ENRICHMENT; NUCLEOSIDES; SEPARATION; RECEPTORS; CAPTURE; SILICA; ACID;

D O I：

10.1039/d3an00297g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Adenosine levels are important in various physiological and pathological activities, but detecting them is difficult because of interference from a complex matrix. This study designed a series of DNA oligomers rich in thymine to enrich adenosine. Their binding affinity (K-d range: 1.25-5.0 mM) to adenosine varied based on the DNA secondary structures, with a clamped hairpin structure showing the highest binding affinity. Compared to other designs, this clamped DNA hairpin underwent the least conformational change during adenosine binding. These DNAs also suppressed the precipitation of supersaturated adenine. Taken together, these results suggest that thymine-rich DNAs could be used to enrich and separate adenosine.

引用

页码：1858 / 1866

页数：9

共 50 条

[21] Oxidatively damaged nucleobases in duplex DNA oligomers: Reaction at thymine-thymine mispairs
Joseph, Joshy
Schuster, Gary B.
Journal of the American Chemical Society, 2009, 131 (39): : 13904 - 13905
[22] Different mechanisms are used by insulin to repress three genes that contain a homologous thymine-rich insulin response element
Patel, S
Lipina, C
Sutherland, C
FEBS LETTERS, 2003, 549 (1-3) : 72 - 76
[23] Increased sequence coverage of thymine-rich oligodeoxynucleotides by infrared multiphoton dissociation compared to collision-induced dissociation
Parr, Carol
Brodbelt, Jennifer S.
JOURNAL OF MASS SPECTROMETRY, 2010, 45 (10): : 1098 - 1103
[24] De novo emergence of adaptive membrane proteins from thymine-rich genomic sequences (vol 11, 781, 2020)
Vakirlis, Nikolaos
Acar, Omer
Hsu, Brian
Coelho, Nelson Castilho
Van Oss, S. Branden
Wacholder, Aaron
Medetgul-Ernar, Kate
Bowman, Ray W., II
Hines, Cameron P.
Iannotta, John
Parikh, Saurin Bipin
McLysaght, Aoife
Camacho, Carlos J.
O'Donnell, Allyson F.
Ideker, Trey
Carvunis, Anne-Ruxandra
NATURE COMMUNICATIONS, 2021, 12 (01)
[25] Thymine-rich single-stranded DNA activates Mcm4/6/7 helicase on Y-fork and bubble-like substrates
You, ZY
Ishimi, Y
Mizuno, T
Sugasawa, K
Hanaoka, F
Masai, H
EMBO JOURNAL, 2003, 22 (22): : 6148 - 6160
[26] Mechanism of oxidative damage of thymine in AT rich DNA sequences
Joseph, Joshy
Schuster, Gary B.
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2011, 241
[27] Conformation-Dependent Exonuclease III Activity Mediated by Metal Ions Reshuffling on Thymine-Rich DNA Duplexes for an Ultrasensitive Electrochemical Method for Hg2+ Detection
Xuan, Feng
Luo, Xiaoteng
Hsing, I-Ming
ANALYTICAL CHEMISTRY, 2013, 85 (09) : 4586 - 4593
[28] Glycogen synthase kinase-3 regulates IGFBP-1 gene transcription through the thymine-rich insulin response element
Finlay, D
Patel, S
Dickson, LM
Shpiro, N
Marquez, R
Rhodes, CJ
Sutherland, C
BMC MOLECULAR BIOLOGY, 2004, 5
[29] INTRASPECIFIC DIVERSITY OF NUCLEOTIDE-SEQUENCES WITHIN THE ADENINE + THYMINE-RICH REGION OF MITOCHONDRIAL-DNA MOLECULES OF DROSOPHILA-MAURITIANA, DROSOPHILA-MELANOGASTER AND DROSOPHILA-SIMULANS
FAURON, CMR
WOLSTENHOLME, DR
NUCLEIC ACIDS RESEARCH, 1980, 8 (22) : 5391 - 5410
[30] Ultrafast vibrational dynamics of adenine-thymine base pairs in DNA oligomers
Dwyer, Jason R.
Szyc, Lukasz
Nibbering, Erik T. J.
Elsaesser, Thomas
JOURNAL OF PHYSICAL CHEMISTRY B, 2008, 112 (36): : 11194 - 11197

← 1 2 3 4 5 →